Experiment set1S462 for Parabacteroides merdae CL09T00C40

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Sucrose 10 mg/mL carbon source

Group: carbon source
Media: Varel_Bryant_medium + Sucrose (10 mg/mL)
Culturing: Pmerdae_CL09T00C40_ML3, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya on 1/10/25
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 16 genes in this experiment

For carbon source Sucrose in Parabacteroides merdae CL09T00C40

For carbon source Sucrose across organisms

SEED Subsystems

Subsystem #Specific
DNA structural proteins, bacterial 1
De Novo Purine Biosynthesis 1
Maltose and Maltodextrin Utilization 1
Predicted carbohydrate hydrolases 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
5-aminoimidazole ribonucleotide biosynthesis I 5 5 1
5-aminoimidazole ribonucleotide biosynthesis II 5 5 1
superpathway of 5-aminoimidazole ribonucleotide biosynthesis 6 6 1
superpathway of purine nucleotides de novo biosynthesis I 21 18 1
superpathway of purine nucleotides de novo biosynthesis II 26 20 1
superpathway of histidine, purine, and pyrimidine biosynthesis 46 38 1