Experiment set1S446 for Parabacteroides merdae CL09T00C40
Mucin from porcine stomach type II 2.5 mg/mL carbon source
Group: carbon sourceMedia: Varel_Bryant_medium + Mucin from porcine stomach,Type II (2.5 mg/mL)
Culturing: Pmerdae_CL09T00C40_ML3, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya on 1/10/25
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Specific Phenotypes
For 1 genes in this experiment
For carbon source Mucin from porcine stomach,Type II in Parabacteroides merdae CL09T00C40
For carbon source Mucin from porcine stomach,Type II across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Glutamate dehydrogenases | 1 |
| Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Glutamate metabolism
- Arginine and proline metabolism
- Nitrogen metabolism
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-glutamate degradation I | 1 | 1 | 1 |
| L-glutamate biosynthesis III | 1 | 1 | 1 |
| L-alanine degradation II (to D-lactate) | 3 | 2 | 1 |
| ethene biosynthesis IV (engineered) | 3 | 1 | 1 |
| L-glutamate and L-glutamine biosynthesis | 7 | 5 | 1 |
| L-glutamate degradation XI (reductive Stickland reaction) | 7 | 2 | 1 |
| 4-aminobutanoate degradation V | 7 | 1 | 1 |
| L-glutamate degradation V (via hydroxyglutarate) | 10 | 2 | 1 |
| methylaspartate cycle | 19 | 10 | 1 |