Experiment set1S381 for Rhodopseudomonas palustris CGA009

Dimethyl sulfide sulfur source

Group: sulfur source control
Media: NoS + Sodium acetate trihydrate (20 mM) + Dimethyl sulfide (1 mM) + Light intensity (30 µmol photons/m2/s from a 60-W incandescent light bulb)
Culturing: RPal_CGA009_ML8, tube, Anaerobic, at 30 (C)
By: Rpal_Fixen on 9/3/24
Media components: 12.5 mM Disodium phosphate, 12.5 mM Potassium phosphate monobasic, 1 g/L Ammonium chloride, 0.1 mM Sodium thiosulfate pentahydrate, 0.002 g/L 4-Aminobenzoic acid, UW concentrated base no sulfur (0.02 g/L Nitrilotriacetic acid, 0.0289 g/L Magnesium chloride hexahydrate, 0.00667 g/L Calcium chloride dihydrate, 1.85e-05 g/L ammonium molybdate tetrahydrate, 0.000698 g/L Iron (II) chloride tetrahydrate, 0.00025 g/L EDTA, 0.001095 g/L Zinc chloride, 0.000154 g/L Manganese (II) chloride tetrahydrate, 3.92e-05 g/L copper (II) chloride dihydrate, 2.5e-05 g/L Cobalt(II) nitrate hexahydrate, 1.77e-05 g/L sodium tetraborate decahydrate)

Specific Phenotypes

For 11 genes in this experiment

For sulfur source control Sodium acetate trihydrate in Rhodopseudomonas palustris CGA009

For sulfur source control Sodium acetate trihydrate across organisms

SEED Subsystems

Subsystem	#Specific
Alkanesulfonate assimilation	3
Alkanesulfonates Utilization	3
Nitrogen fixation	3
Putative sulfate assimilation cluster	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Tetrachloroethene degradation
• Nitrogen metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
nitrogen fixation I (ferredoxin)	1	1	1
two-component alkanesulfonate monooxygenase	2	1	1
S-methyl-5-thio-α-D-ribose 1-phosphate degradation III	5	3	1
dimethyl sulfide degradation II (oxidation)	5	2	1