Experiment set1S366 for Rhodopseudomonas palustris CGA009

Taurine sulfur source

Group: sulfur source
Media: NoS + L-Malic acid disodium salt (10 mM) + Taurine (1 mM) + Light intensity (30 µmol photons/m2/s from a 60-W incandescent light bulb)
Culturing: RPal_CGA009_ML8, tube, Anaerobic, at 30 (C)
By: Rpal_Fixen on 9/2/24
Media components: 12.5 mM Disodium phosphate, 12.5 mM Potassium phosphate monobasic, 1 g/L Ammonium chloride, 0.1 mM Sodium thiosulfate pentahydrate, 0.002 g/L 4-Aminobenzoic acid, UW concentrated base no sulfur (0.02 g/L Nitrilotriacetic acid, 0.0289 g/L Magnesium chloride hexahydrate, 0.00667 g/L Calcium chloride dihydrate, 1.85e-05 g/L ammonium molybdate tetrahydrate, 0.000698 g/L Iron (II) chloride tetrahydrate, 0.00025 g/L EDTA, 0.001095 g/L Zinc chloride, 0.000154 g/L Manganese (II) chloride tetrahydrate, 3.92e-05 g/L copper (II) chloride dihydrate, 2.5e-05 g/L Cobalt(II) nitrate hexahydrate, 1.77e-05 g/L sodium tetraborate decahydrate)

Specific Phenotypes

For 6 genes in this experiment

For sulfur source L-Malic acid disodium salt in Rhodopseudomonas palustris CGA009

For sulfur source L-Malic acid disodium salt across organisms

SEED Subsystems

Subsystem	#Specific
Alkanesulfonate assimilation	3
Alkanesulfonates Utilization	3
Putative sulfate assimilation cluster	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Tetrachloroethene degradation
• Nitrogen metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
nitrogen fixation I (ferredoxin)	1	1	1