Experiment set1S355 for Rhodopseudomonas palustris CGA009

2-(Methylthio)ethanol sulfur source

Group: sulfur source
Media: NoS + L-Malic acid disodium salt (10 mM) + 2-(Methylthio)ethanol (1 mM) + Light intensity (30 µmol photons/m2/s from a 60-W incandescent light bulb)
Culturing: RPal_CGA009_ML8, tube, Anaerobic, at 30 (C)
By: Rpal_Fixen on 9/2/24
Media components: 12.5 mM Disodium phosphate, 12.5 mM Potassium phosphate monobasic, 1 g/L Ammonium chloride, 0.1 mM Sodium thiosulfate pentahydrate, 0.002 g/L 4-Aminobenzoic acid, UW concentrated base no sulfur (0.02 g/L Nitrilotriacetic acid, 0.0289 g/L Magnesium chloride hexahydrate, 0.00667 g/L Calcium chloride dihydrate, 1.85e-05 g/L ammonium molybdate tetrahydrate, 0.000698 g/L Iron (II) chloride tetrahydrate, 0.00025 g/L EDTA, 0.001095 g/L Zinc chloride, 0.000154 g/L Manganese (II) chloride tetrahydrate, 3.92e-05 g/L copper (II) chloride dihydrate, 2.5e-05 g/L Cobalt(II) nitrate hexahydrate, 1.77e-05 g/L sodium tetraborate decahydrate)

Specific Phenotypes

For 1 genes in this experiment

For sulfur source L-Malic acid disodium salt in Rhodopseudomonas palustris CGA009

For sulfur source L-Malic acid disodium salt across organisms

SEED Subsystems

Subsystem	#Specific
Entner-Doudoroff Pathway	1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	1
Methylglyoxal Metabolism	1
Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycolysis / Gluconeogenesis
• Ascorbate and aldarate metabolism
• Fatty acid metabolism
• Urea cycle and metabolism of amino groups
• Valine, leucine and isoleucine degradation
• Lysine degradation
• Arginine and proline metabolism
• Histidine metabolism
• Tryptophan metabolism
• beta-Alanine metabolism
• Glycerolipid metabolism
• Pyruvate metabolism
• 1,2-Dichloroethane degradation
• Propanoate metabolism
• 3-Chloroacrylic acid degradation
• Butanoate metabolism
• Limonene and pinene degradation

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
ethylene glycol degradation	2	1	1
putrescine degradation V	2	1	1
putrescine degradation I	2	1	1
ethanol degradation IV	3	3	1
ethanol degradation II	3	3	1
ethanol degradation III	3	2	1
putrescine degradation IV	3	2	1
hypotaurine degradation	3	2	1
histamine degradation	3	1	1
phytol degradation	4	3	1
fatty acid α-oxidation I (plants)	4	2	1
putrescine degradation III	4	2	1
D-arabinose degradation II	4	2	1
L-tryptophan degradation X (mammalian, via tryptamine)	4	2	1
mitochondrial NADPH production (yeast)	5	3	1
sphingosine and sphingosine-1-phosphate metabolism	10	4	2
dopamine degradation	5	2	1
octane oxidation	5	2	1
3-methyl-branched fatty acid α-oxidation	6	3	1
alkane oxidation	6	1	1
noradrenaline and adrenaline degradation	13	8	2
serotonin degradation	7	4	1
superpathway of glycol metabolism and degradation	7	4	1
ceramide degradation by α-oxidation	7	2	1
limonene degradation IV (anaerobic)	7	2	1
superpathway of NAD/NADP - NADH/NADPH interconversion (yeast)	8	5	1
aromatic biogenic amine degradation (bacteria)	8	3	1
superpathway of ornithine degradation	8	3	1
ceramide and sphingolipid recycling and degradation (yeast)	16	4	2
Entner-Doudoroff pathway II (non-phosphorylative)	9	4	1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation	11	3	1
superpathway of L-arginine and L-ornithine degradation	13	5	1
superpathway of pentose and pentitol degradation	42	9	1