Experiment set1S331 for Rhodopseudomonas palustris CGA009

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L-Leucine carbon source; anaerobic

Group: carbon source
Media: PM + L-Leucine (4.5 mM) + Sodium bicarbonate (10 mM) + Light intensity (30 µmol photons/m2/s from a 60-W incandescent light bulb)
Culturing: RPal_CGA009_ML8, tube, Anaerobic, at 30 (C)
By: Rpal_Yasu on 9/1/24
Media components: 12.5 mM Disodium phosphate, 12.5 mM Potassium phosphate monobasic, 1 g/L Ammonium Sulfate, 0.1 mM Sodium thiosulfate pentahydrate, 0.002 g/L 4-Aminobenzoic acid, UW concentrated base (0.02 g/L Nitrilotriacetic acid, 0.0289 g/L Magnesium sulfate, 0.00667 g/L Calcium chloride dihydrate, 1.85e-05 g/L ammonium molybdate tetrahydrate, 0.000698 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L EDTA, 0.001095 g/L Zinc sulfate heptahydrate, 0.000154 g/L Manganese (II) sulfate monohydrate, 3.92e-05 g/L Copper (II) sulfate pentahydrate, 2.5e-05 g/L Cobalt(II) nitrate hexahydrate, 1.77e-05 g/L sodium tetraborate decahydrate)

Specific Phenotypes

For 16 genes in this experiment

For carbon source L-Leucine in Rhodopseudomonas palustris CGA009

For carbon source L-Leucine across organisms

SEED Subsystems

Subsystem #Specific
ABC transporter branched-chain amino acid (TC 3.A.1.4.1) 5
Leucine Degradation and HMG-CoA Metabolism 3
Serine-glyoxylate cycle 3
HMG CoA Synthesis 2
Aromatic amino acid interconversions with aryl acids 1
Catechol branch of beta-ketoadipate pathway 1
Glutaredoxins 1
Glutathione: Redox cycle 1
Homogentisate pathway of aromatic compound degradation 1
Lysine degradation 1
Protocatechuate branch of beta-ketoadipate pathway 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
ketolysis 3 3 1
L-leucine degradation I 6 5 2
TCA cycle VI (Helicobacter) 9 8 1