Experiment set1S322 for Rhodopseudomonas palustris CGA009
Citraconic acid carbon source; anaerobic
Group: carbon sourceMedia: PM + Citraconic acid (10 mM) + Sodium bicarbonate (10 mM) + Light intensity (30 µmol photons/m2/s from a 60-W incandescent light bulb)
Culturing: RPal_CGA009_ML8, tube, Anaerobic, at 30 (C)
By: Rpal_Yasu on 9/1/24
Media components: 12.5 mM Disodium phosphate, 12.5 mM Potassium phosphate monobasic, 1 g/L Ammonium Sulfate, 0.1 mM Sodium thiosulfate pentahydrate, 0.002 g/L 4-Aminobenzoic acid, UW concentrated base (0.02 g/L Nitrilotriacetic acid, 0.0289 g/L Magnesium sulfate, 0.00667 g/L Calcium chloride dihydrate, 1.85e-05 g/L ammonium molybdate tetrahydrate, 0.000698 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L EDTA, 0.001095 g/L Zinc sulfate heptahydrate, 0.000154 g/L Manganese (II) sulfate monohydrate, 3.92e-05 g/L Copper (II) sulfate pentahydrate, 2.5e-05 g/L Cobalt(II) nitrate hexahydrate, 1.77e-05 g/L sodium tetraborate decahydrate)
Specific Phenotypes
For 10 genes in this experiment
For carbon source Citraconic acid in Rhodopseudomonas palustris CGA009
For carbon source Citraconic acid across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Homogentisate pathway of aromatic compound degradation | 3 |
| Aromatic amino acid interconversions with aryl acids | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Valine, leucine and isoleucine degradation
- Lipopolysaccharide biosynthesis
- Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
- Propanoate metabolism
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| propanoyl CoA degradation I | 3 | 3 | 1 |
| 2-oxobutanoate degradation I | 4 | 3 | 1 |
| propane degradation I | 4 | 1 | 1 |
| pyruvate fermentation to propanoate I | 7 | 4 | 1 |
| anaerobic energy metabolism (invertebrates, mitochondrial) | 10 | 8 | 1 |
| L-glutamate degradation VIII (to propanoate) | 11 | 4 | 1 |
| ethylmalonyl-CoA pathway | 11 | 3 | 1 |
| 3-hydroxypropanoate cycle | 13 | 9 | 1 |
| (S)-lactate fermentation to propanoate, acetate and hydrogen | 13 | 6 | 1 |
| crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) | 14 | 3 | 1 |
| superpathway of L-methionine salvage and degradation | 16 | 10 | 1 |
| superpathway of anaerobic energy metabolism (invertebrates) | 17 | 13 | 1 |
| 3-hydroxypropanoate/4-hydroxybutanate cycle | 18 | 12 | 1 |
| superpathway of the 3-hydroxypropanoate cycle | 18 | 9 | 1 |
| methylaspartate cycle | 19 | 11 | 1 |