Experiment set1S296 for Rhodopseudomonas palustris CGA009
starvation expt 2; 3 days; outgrowth in CAAc
Group: survivalMedia: PM_acetate + Light intensity (30 µmol photons/m2/s from a 60-W incandescent light bulb)
Culturing: RPal_CGA009_ML8, tube, Anaerobic
By: Rpal_Yasu on 9/1/24
Media components: 12.5 mM Disodium phosphate, 12.5 mM Potassium phosphate monobasic, 1 g/L Ammonium Sulfate, 0.1 mM Sodium thiosulfate pentahydrate, 0.002 g/L 4-Aminobenzoic acid, 10 mM Sodium acetate, UW concentrated base (0.02 g/L Nitrilotriacetic acid, 0.0289 g/L Magnesium sulfate, 0.00667 g/L Calcium chloride dihydrate, 1.85e-05 g/L ammonium molybdate tetrahydrate, 0.000698 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L EDTA, 0.001095 g/L Zinc sulfate heptahydrate, 0.000154 g/L Manganese (II) sulfate monohydrate, 3.92e-05 g/L Copper (II) sulfate pentahydrate, 2.5e-05 g/L Cobalt(II) nitrate hexahydrate, 1.77e-05 g/L sodium tetraborate decahydrate)
Specific Phenotypes
For 3 genes in this experiment
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Pyridoxin (Vitamin B6) Biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| pyridoxal 5'-phosphate salvage I | 6 | 5 | 2 |
| pyridoxal 5'-phosphate salvage II (plants) | 9 | 8 | 2 |
| superpathway of pyridoxal 5'-phosphate biosynthesis and salvage | 12 | 10 | 2 |
| pyridoxal 5'-phosphate biosynthesis I | 7 | 6 | 1 |
| 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis II | 8 | 4 | 1 |