Experiment set1S230 for Mycobacterium tuberculosis H37Rv

L-Asparagine carbon source

Group: carbon source
Media: Sautons minimal media with no carbon + L-Asparagine (10 mM)
Culturing: MycoTube_ML10, tube, Aerobic, at 37 (C)
Growth: about 3.4 generations
By: Kayla Dinshaw on 6/30/23
Media components: 0.8 g/L Sodium Chloride, 2.5 g/L Disodium phosphate, 1 g/L Potassium phosphate monobasic, 0.2 g/L L-Asparagine, 0.05 g/L Ferric ammonium citrate, 0.02 g/L Magnesium sulfate, 0.0005 g/L Calcium chloride, 1e-05 g/L Zinc sulfate heptahydrate, 0.2 vol% Ethanol, 0.05 vol% Tyloxapol

Specific Phenotypes

For 14 genes in this experiment

For carbon source L-Asparagine in Mycobacterium tuberculosis H37Rv

For carbon source L-Asparagine across organisms

SEED Subsystems

Subsystem	#Specific
Respiratory Complex I	10

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Oxidative phosphorylation
• Ubiquinone and menaquinone biosynthesis
• Histidine metabolism
• Tyrosine metabolism
• Tryptophan metabolism
• Benzoxazinone biosynthesis
• Naphthalene and anthracene degradation
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Phenylpropanoid biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
NADH to cytochrome bo oxidase electron transfer I	2	1	1
NADH to cytochrome bd oxidase electron transfer I	2	1	1
aerobic respiration III (alternative oxidase pathway)	3	2	1
aerobic respiration I (cytochrome c)	4	3	1
coenzyme B/coenzyme M regeneration I (methanophenazine-dependent)	4	1	1
NAD(P)/NADPH interconversion	6	3	1
Fe(II) oxidation	6	2	1