Experiment set1S229 for Mycobacterium tuberculosis H37Rv
L-Asparagine carbon source
Group: carbon sourceMedia: Sautons minimal media with no carbon + L-Asparagine (10 mM)
Culturing: MycoTube_ML10, tube, Aerobic, at 37 (C)
Growth: about 3.4 generations
By: Kayla Dinshaw on 6/30/23
Media components: 0.8 g/L Sodium Chloride, 2.5 g/L Disodium phosphate, 1 g/L Potassium phosphate monobasic, 0.2 g/L L-Asparagine, 0.05 g/L Ferric ammonium citrate, 0.02 g/L Magnesium sulfate, 0.0005 g/L Calcium chloride, 1e-05 g/L Zinc sulfate heptahydrate, 0.2 vol% Ethanol, 0.05 vol% Tyloxapol
Specific Phenotypes
For 6 genes in this experiment
For carbon source L-Asparagine in Mycobacterium tuberculosis H37Rv
For carbon source L-Asparagine across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Respiratory Complex I | 5 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| NADH to cytochrome bd oxidase electron transfer I | 2 | 1 | 1 |
| NADH to cytochrome bo oxidase electron transfer I | 2 | 1 | 1 |
| aerobic respiration III (alternative oxidase pathway) | 3 | 2 | 1 |
| aerobic respiration I (cytochrome c) | 4 | 3 | 1 |
| coenzyme B/coenzyme M regeneration I (methanophenazine-dependent) | 4 | 1 | 1 |
| NAD(P)/NADPH interconversion | 6 | 3 | 1 |
| Fe(II) oxidation | 6 | 2 | 1 |