Experiment set1S223 for Mycobacterium tuberculosis H37Rv

Potassium acetate carbon source

Group: carbon source
Media: Sautons minimal media with no carbon + Potassium acetate (10 mM)
Culturing: MycoTube_ML10, tube, Aerobic, at 37 (C)
Growth: about 3.4 generations
By: Kayla Dinshaw on 6/30/23
Media components: 0.8 g/L Sodium Chloride, 2.5 g/L Disodium phosphate, 1 g/L Potassium phosphate monobasic, 0.2 g/L L-Asparagine, 0.05 g/L Ferric ammonium citrate, 0.02 g/L Magnesium sulfate, 0.0005 g/L Calcium chloride, 1e-05 g/L Zinc sulfate heptahydrate, 0.2 vol% Ethanol, 0.05 vol% Tyloxapol

Specific Phenotypes

For 2 genes in this experiment

For carbon source Potassium acetate in Mycobacterium tuberculosis H37Rv

For carbon source Potassium acetate across organisms

SEED Subsystems

Subsystem	#Specific
Ethanolamine utilization	1
Fermentations: Lactate	1
Fermentations: Mixed acid	1
MLST	1
Propanediol utilization	1
Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate	1
Threonine anaerobic catabolism gene cluster	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Ubiquinone and menaquinone biosynthesis
• Histidine metabolism
• Tyrosine metabolism
• Tryptophan metabolism
• Benzoxazinone biosynthesis
• Taurine and hypotaurine metabolism
• Pyruvate metabolism
• Naphthalene and anthracene degradation
• Propanoate metabolism
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Phenylpropanoid biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
acetate and ATP formation from acetyl-CoA I	2	2	1
sulfoacetaldehyde degradation I	2	1	1
superpathway of acetate utilization and formation	3	3	1
pyruvate fermentation to acetate II	3	2	1
pyruvate fermentation to acetate IV	3	2	1
pyruvate fermentation to acetate VII	3	2	1
pyruvate fermentation to acetate I	3	2	1
pyruvate fermentation to acetate and (S)-lactate I	4	2	1
pyruvate fermentation to acetate and lactate II	4	2	1
sulfolactate degradation II	4	1	1
acetylene degradation (anaerobic)	5	4	1
ethanolamine utilization	5	4	1
(S)-propane-1,2-diol degradation	5	4	1
L-threonine degradation I	6	5	1
superpathway of taurine degradation	6	3	1
methanogenesis from acetate	6	2	1
superpathway of sulfolactate degradation	6	2	1
acetyl-CoA fermentation to butanoate	7	4	1
lactate fermentation to acetate, CO2 and hydrogen (Desulfovibrionales)	8	4	1
superpathway of Clostridium acetobutylicum acidogenic fermentation	9	6	1
superpathway of fermentation (Chlamydomonas reinhardtii)	9	5	1
superpathway of L-alanine fermentation (Stickland reaction)	9	3	1
L-lysine fermentation to acetate and butanoate	10	4	1
gallate degradation III (anaerobic)	11	4	1
(S)-lactate fermentation to propanoate, acetate and hydrogen	13	8	1
mixed acid fermentation	16	13	1
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation	17	8	1
heterolactic fermentation	18	13	1
superpathway of L-threonine metabolism	18	12	1
hexitol fermentation to lactate, formate, ethanol and acetate	19	16	1
superpathway of methanogenesis	21	2	1
superpathway of N-acetylneuraminate degradation	22	16	1
purine nucleobases degradation II (anaerobic)	24	12	1
superpathway of L-lysine degradation	43	10	1