Experiment set1S221 for Desulfovibrio vulgaris Hildenborough JW710
MoLS4 with L-Methionine (10mM) as nutrient
Group: nutrientMedia: MoLS4 + L-Methionine (10 mM), pH=7.2
Culturing: DvH_JW710, 24 deep-well microplate, Anaerobic, at 30 (C), shaken=0 rpm
By: Valentine on 4/6/2016
Media components: 30 mM Sodium sulfate, 60 mM Sodium D,L-Lactate, 20 mM Ammonium chloride, 30 mM Tris hydrochloride, 0.12 mM EDTA, 1 mM Sodium sulfide nonahydrate, 8 mM Magnesium chloride hexahydrate, 0.6 mM Calcium chloride, 2 mM Potassium phosphate dibasic, 60 uM Iron (II) chloride tetrahydrate, Desulfovibrio trace elements (15 uM Manganese (II) chloride tetrahydrate, 7.8 uM Cobalt chloride hexahydrate, 9 uM Zinc chloride, 1.26 uM Sodium molybdate, 1.92 uM Boric Acid, 2.28 uM Nickel (II) sulfate hexahydrate, 0.06 uM copper (II) chloride dihydrate, 0.21 uM Sodium selenite pentahydrate, 0.144 uM Sodium tungstate dihydrate), Thauer's vitamin mix (0.01 mg/L Pyridoxine HCl, 0.005 mg/L 4-Aminobenzoic acid, 0.005 mg/L Lipoic acid, 0.005 mg/L Nicotinic Acid, 0.005 mg/L Riboflavin, 0.005 mg/L Thiamine HCl, 0.005 mg/L calcium pantothenate, 0.002 mg/L biotin, 0.002 mg/L Folic Acid, 0.0001 mg/L Cyanocobalamin, 0.2 mg/L Choline chloride)
Specific Phenotypes
For 1 genes in this experiment
For nutrient L-Methionine in Desulfovibrio vulgaris Hildenborough JW710
For nutrient L-Methionine across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Thiamin biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| thiamine diphosphate salvage V | 3 | 3 | 1 |
| prenylated FMNH2 biosynthesis | 3 | 1 | 1 |
| thiamine diphosphate salvage II | 5 | 5 | 1 |
| thiamine diphosphate salvage IV (yeast) | 7 | 4 | 1 |
| thiamine diphosphate formation from pyrithiamine and oxythiamine (yeast) | 8 | 4 | 1 |