Experiment set1S176 for Synechocystis sp000284455 PCC 6803

Compare to:

growth in full media bubbled with ambient air

Group: carbon dioxide
Media: BG11C, pH=8
Culturing: Synechocystis_PCC6803_ML3, vertical flask, air bubling, growth chamber, continous light, at 30 (C), shaken=air bubling
Growth: about 5.0 generations
By: Luis on 3/17/25
Media components: 17.6 mM Sodium nitrate, 0.2 mM Potassium phosphate dibasic, 0.3 mM Magnesium Sulfate Heptahydrate, 0.24 mM Calcium chloride dihydrate, 28.5 uM Citric Acid, 0.006 g/L Ferric ammonium citrate, 2.4 uM EDTA (disodium salt), 0.2 mM Sodium bicarbonate, Trace metal mix A5 (0.00286 g/L Boric Acid, 0.00181 g/L Manganese (II) chloride tetrahydrate, 0.000222 g/L Zinc sulfate heptahydrate, 0.00039 g/L Sodium Molybdate Dihydrate, 7.9e-05 g/L Copper (II) sulfate pentahydrate, 0.0494 mg/L Cobalt(II) nitrate hexahydrate)

Specific Phenotypes

For 9 genes in this experiment

SEED Subsystems

Subsystem #Specific
CO2 uptake, carboxysome 1
Serine-glyoxylate cycle 1
TCA Cycle 1
Transcription initiation, bacterial sigma factors 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
pyruvate fermentation to acetate VI 3 2 1
pyruvate fermentation to acetate V 3 2 1
adipate biosynthesis 5 1 1
adipate degradation 5 1 1
TCA cycle VIII (Chlamydia) 6 6 1
incomplete reductive TCA cycle 7 4 1
TCA cycle II (plants and fungi) 9 8 1
TCA cycle V (2-oxoglutarate synthase) 9 8 1
TCA cycle I (prokaryotic) 10 9 1
TCA cycle III (animals) 10 8 1
anaerobic energy metabolism (invertebrates, mitochondrial) 10 5 1
reductive TCA cycle I 11 7 1
superpathway of glyoxylate bypass and TCA 12 9 1
reductive TCA cycle II 12 6 1
superpathway of anaerobic energy metabolism (invertebrates) 17 9 1
methylaspartate cycle 19 9 1
superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle 22 19 1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass 26 21 1
Methanobacterium thermoautotrophicum biosynthetic metabolism 56 20 1