Experiment set1S175 for Synechocystis sp000284455 PCC 6803

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growth in media lacking HCO3 buffered with 10 TES pH8 and bubbled with air enriched with 5% CO2

Group: carbon dioxide
Media: BG11_TES_no_bicarb, pH=8
Culturing: Synechocystis_PCC6803_ML3, vertical flask, 5% CO2 enriched air bubling, growth chamber, continous light, at 30 (C), shaken=air bubling
Growth: about 4.0 generations
By: Luis on 3/17/25
Media components: 17.6 mM Sodium nitrate, 0.2 mM Potassium phosphate dibasic, 0.3 mM Magnesium Sulfate Heptahydrate, 0.24 mM Calcium chloride dihydrate, 28.5 uM Citric Acid, 0.006 g/L Ferric ammonium citrate, 2.4 uM EDTA (disodium salt), 20 mM TES, Trace metal mix A5 (0.00286 g/L Boric Acid, 0.00181 g/L Manganese (II) chloride tetrahydrate, 0.000222 g/L Zinc sulfate heptahydrate, 0.00039 g/L Sodium Molybdate Dihydrate, 7.9e-05 g/L Copper (II) sulfate pentahydrate, 0.0494 mg/L Cobalt(II) nitrate hexahydrate)

Specific Phenotypes

For 8 genes in this experiment

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes: