Experiment set1S161 for Synechocystis sp000284455 PCC 6803
growth in full media supplemented with 3 .M Cl2Co
Group: metal stressMedia: BG11C + Cobalt chloride hexahydrate (3 uM)
Culturing: Synechocystis_PCC6803_ML3, flask, 100 rpm orbital shaking, growth chamber, continous light, at 30 (C), shaken=100 rpm
Growth: about 5.0 generations
By: Luis on 3/3/25
Media components: 17.6 mM Sodium nitrate, 0.2 mM Potassium phosphate dibasic, 0.3 mM Magnesium Sulfate Heptahydrate, 0.24 mM Calcium chloride dihydrate, 28.5 uM Citric Acid, 0.006 g/L Ferric ammonium citrate, 2.4 uM EDTA (disodium salt), 0.2 mM Sodium bicarbonate, Trace metal mix A5 (0.00286 g/L Boric Acid, 0.00181 g/L Manganese (II) chloride tetrahydrate, 0.000222 g/L Zinc sulfate heptahydrate, 0.00039 g/L Sodium Molybdate Dihydrate, 7.9e-05 g/L Copper (II) sulfate pentahydrate, 0.0494 mg/L Cobalt(II) nitrate hexahydrate)
Specific Phenotypes
For 3 genes in this experiment
For metal stress Cobalt chloride hexahydrate in Synechocystis sp000284455 PCC 6803
For metal stress Cobalt chloride hexahydrate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Cobalamin synthesis | 1 |
| Cobalt-zinc-cadmium resistance | 1 |
| Coenzyme B12 biosynthesis | 1 |
| Photosystem I | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| cyclic electron flow | 3 | 2 | 1 |
| photosynthesis light reactions | 4 | 4 | 1 |
| superpathway of photosynthetic hydrogen production | 6 | 5 | 1 |
| cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation) | 13 | 11 | 1 |
| cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) | 15 | 14 | 1 |
| oxygenic photosynthesis | 17 | 17 | 1 |
| ethene biosynthesis V (engineered) | 25 | 24 | 1 |
| photosynthetic 3-hydroxybutanoate biosynthesis (engineered) | 26 | 23 | 1 |
| 1-butanol autotrophic biosynthesis (engineered) | 27 | 22 | 1 |
| adenosylcobalamin biosynthesis II (aerobic) | 33 | 28 | 1 |
| adenosylcobalamin biosynthesis I (anaerobic) | 36 | 31 | 1 |