Experiment set1S144 for Synechocystis sp000284455 PCC 6803
Arg 10 mM as N source, buffered with 20 mM TES pH8
Group: nitrogen sourceMedia: BG11C_TES + L-Arginine (10 mM)
Culturing: Synechocystis_PCC6803_ML3, flask, 100 rpm orbital shaking, growth chamber, continous light, at 30 (C), shaken=100 rpm
Growth: about 5.0 generations
By: Luis on 1/20/25
Media components: 17.6 mM Sodium nitrate, 0.2 mM Potassium phosphate dibasic, 0.3 mM Magnesium Sulfate Heptahydrate, 0.24 mM Calcium chloride dihydrate, 28.5 uM Citric Acid, 0.006 g/L Ferric ammonium citrate, 2.4 uM EDTA (disodium salt), 0.2 mM Sodium bicarbonate, 20 mM TES, Trace metal mix A5 (0.00286 g/L Boric Acid, 0.00181 g/L Manganese (II) chloride tetrahydrate, 0.000222 g/L Zinc sulfate heptahydrate, 0.00039 g/L Sodium Molybdate Dihydrate, 7.9e-05 g/L Copper (II) sulfate pentahydrate, 0.0494 mg/L Cobalt(II) nitrate hexahydrate)
Specific Phenotypes
For 3 genes in this experiment
For nitrogen source L-Arginine in Synechocystis sp000284455 PCC 6803
For nitrogen source L-Arginine across organisms
Metabolic Maps
Color code by fitness: see overview map or list of maps.
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-proline biosynthesis II (from arginine) | 2 | 2 | 2 |
| L-ornithine degradation I (L-proline biosynthesis) | 1 | 1 | 1 |
| L-arginine degradation VII (arginase 3 pathway) | 2 | 1 | 1 |