growth in full media and 10 mM glucose= photoheterotrophic growth
Group:
nitrogen source
Media:
BG11C +
D-Glucose (10 mM) + DCMU (20 uM)
Culturing: Synechocystis_PCC6803_ML3, flask, 100 rpm orbital shaking, growth chamber, continous light, at 30 (C), shaken=100 rpm
Growth: about 4.0
generations
By: Luis on
1/20/25
Specific Phenotypes
For 4 genes in this experiment
For nitrogen source D-Glucose in Synechocystis sp000284455 PCC 6803
For nitrogen source D-Glucose across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|
| NADP biosynthesis | 1 | 1 | 1 |
| NAD phosphorylation and transhydrogenation | 2 | 2 | 1 |
| malate/L-aspartate shuttle pathway | 2 | 2 | 1 |
| 4-aminobutanoate degradation III | 2 | 2 | 1 |
| 4-aminobutanoate degradation I | 2 | 2 | 1 |
| cytosolic NADPH production (yeast) | 5 | 3 | 2 |
| pentose phosphate pathway (oxidative branch) I | 3 | 3 | 1 |
| superpathway of 4-aminobutanoate degradation | 3 | 2 | 1 |
| NAD phosphorylation and dephosphorylation | 3 | 2 | 1 |
| 4-aminobutanoate degradation IV | 3 | 1 | 1 |
| N-methylpyrrolidone degradation | 3 | 1 | 1 |
| GABA shunt II | 4 | 4 | 1 |
| GABA shunt I | 4 | 3 | 1 |
| superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) | 8 | 4 | 2 |
| mitochondrial NADPH production (yeast) | 5 | 2 | 1 |
| TCA cycle VIII (Chlamydia) | 6 | 6 | 1 |
| glyoxylate cycle | 6 | 4 | 1 |
| NAD(P)/NADPH interconversion | 6 | 3 | 1 |
| formaldehyde oxidation I | 6 | 3 | 1 |
| L-lysine biosynthesis VI | 7 | 7 | 1 |
| anaerobic energy metabolism (invertebrates, cytosol) | 7 | 4 | 1 |
| incomplete reductive TCA cycle | 7 | 4 | 1 |
| pyruvate fermentation to propanoate I | 7 | 2 | 1 |
| pentose phosphate pathway | 8 | 8 | 1 |
| TCA cycle V (2-oxoglutarate synthase) | 9 | 8 | 1 |
| TCA cycle IV (2-oxoglutarate decarboxylase) | 9 | 8 | 1 |
| TCA cycle II (plants and fungi) | 9 | 8 | 1 |
| L-lysine biosynthesis I | 9 | 7 | 1 |
| Entner-Doudoroff pathway I | 9 | 7 | 1 |
| L-lysine biosynthesis II | 9 | 6 | 1 |
| TCA cycle I (prokaryotic) | 10 | 9 | 1 |
| TCA cycle III (animals) | 10 | 8 | 1 |
| reductive TCA cycle I | 11 | 7 | 1 |
| C4 photosynthetic carbon assimilation cycle, NAD-ME type | 11 | 6 | 1 |
| superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation | 11 | 5 | 1 |
| L-glutamate degradation VIII (to propanoate) | 11 | 2 | 1 |
| superpathway of glyoxylate bypass and TCA | 12 | 9 | 1 |
| gluconeogenesis III | 12 | 8 | 1 |
| reductive TCA cycle II | 12 | 6 | 1 |
| gluconeogenesis I | 13 | 12 | 1 |
| formaldehyde assimilation I (serine pathway) | 13 | 8 | 1 |
| superpathway of L-arginine and L-ornithine degradation | 13 | 6 | 1 |
| (S)-lactate fermentation to propanoate, acetate and hydrogen | 13 | 4 | 1 |
| superpathway of glyoxylate cycle and fatty acid degradation | 14 | 7 | 1 |
| superpathway of L-lysine, L-threonine and L-methionine biosynthesis II | 15 | 12 | 1 |
| mixed acid fermentation | 16 | 12 | 1 |
| superpathway of glycolysis and the Entner-Doudoroff pathway | 17 | 14 | 1 |
| superpathway of glucose and xylose degradation | 17 | 14 | 1 |
| superpathway of anaerobic energy metabolism (invertebrates) | 17 | 9 | 1 |
| heterolactic fermentation | 18 | 14 | 1 |
| superpathway of L-lysine, L-threonine and L-methionine biosynthesis I | 18 | 12 | 1 |
| methylaspartate cycle | 19 | 9 | 1 |
| superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle | 22 | 19 | 1 |
| aspartate superpathway | 25 | 19 | 1 |
| superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass | 26 | 21 | 1 |
| Methanobacterium thermoautotrophicum biosynthetic metabolism | 56 | 20 | 1 |