Experiment set1S141 for Synechocystis sp000284455 PCC 6803

Compare to:

NaNO2 10 mM as N source, buffered with 20 mM TES pH8

Group: nitrogen source
Media: BG11C_TES + Sodium nitrite (10 mM)
Culturing: Synechocystis_PCC6803_ML3, flask, 100 rpm orbital shaking, growth chamber, continous light, at 30 (C), shaken=100 rpm
Growth: about 3.0 generations
By: Luis on 1/20/25
Media components: 17.6 mM Sodium nitrate, 0.2 mM Potassium phosphate dibasic, 0.3 mM Magnesium Sulfate Heptahydrate, 0.24 mM Calcium chloride dihydrate, 28.5 uM Citric Acid, 0.006 g/L Ferric ammonium citrate, 2.4 uM EDTA (disodium salt), 0.2 mM Sodium bicarbonate, 20 mM TES, Trace metal mix A5 (0.00286 g/L Boric Acid, 0.00181 g/L Manganese (II) chloride tetrahydrate, 0.000222 g/L Zinc sulfate heptahydrate, 0.00039 g/L Sodium Molybdate Dihydrate, 7.9e-05 g/L Copper (II) sulfate pentahydrate, 0.0494 mg/L Cobalt(II) nitrate hexahydrate)

Specific Phenotypes

For 6 genes in this experiment

For nitrogen source Sodium nitrite in Synechocystis sp000284455 PCC 6803

For nitrogen source Sodium nitrite across organisms

SEED Subsystems

Subsystem #Specific
Folate Biosynthesis 1
Queuosine-Archaeosine Biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
pyrimidine nucleobases salvage II 2 2 1
pyrimidine ribonucleosides salvage III 2 1 1