Experiment set1IT093 for Pseudomonas fluorescens FW300-N2E3
LB with copper (II) chloride dihydrate 2.5 mM
Group: stressMedia: LB + copper (II) chloride dihydrate (2.5 mM), pH=7
Culturing: pseudo3_N2E3_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 28 (C), shaken=200 rpm
By: Jayashree on 4/28/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 892 B7,B8
Specific Phenotypes
For 10 genes in this experiment
For stress copper (II) chloride dihydrate in Pseudomonas fluorescens FW300-N2E3
For stress copper (II) chloride dihydrate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Copper homeostasis | 2 |
| Biogenesis of c-type cytochromes | 1 |
| Lysine degradation | 1 |
| Orphan regulatory proteins | 1 |
| Periplasmic disulfide interchange | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| thiosulfate disproportionation IV (rhodanese) | 1 | 1 | 1 |
| L-cysteine degradation III | 2 | 1 | 1 |
| sulfide oxidation IV (mitochondria) | 5 | 2 | 1 |