Experiment set1IT090 for Pseudomonas fluorescens FW300-N2E3

LB with Nickel (II) chloride hexahydrate 0.625 mM

Group: stress
Media: LB + Nickel (II) chloride hexahydrate (0.625 mM), pH=7
Culturing: pseudo3_N2E3_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 28 (C), shaken=200 rpm
By: Jayashree on 4/28/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 892 A3,A4

Specific Phenotypes

For 11 genes in this experiment

For stress Nickel (II) chloride hexahydrate in Pseudomonas fluorescens FW300-N2E3

For stress Nickel (II) chloride hexahydrate across organisms

SEED Subsystems

Subsystem	#Specific
Cobalt-zinc-cadmium resistance	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Histidine metabolism
• Biosynthesis of alkaloids derived from histidine and purine

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
histamine biosynthesis	1	1	1