Experiment set1IT089 for Bacteroides thetaiotaomicron VPI-5482
N-Acetyl-D-Glucosamine carbon source
Group: carbon sourceMedia: Varel_Bryant_medium + N-Acetyl-D-Glucosamine (20 mM)
Culturing: Btheta_ML6, 24-well transparent microplate, Anaerobic, at 37 (C), shaken=0 rpm
By: Hualan on 8-May-17
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Specific Phenotypes
For 2 genes in this experiment
For carbon source N-Acetyl-D-Glucosamine in Bacteroides thetaiotaomicron VPI-5482
For carbon source N-Acetyl-D-Glucosamine across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Entner-Doudoroff Pathway | 1 |
| Glycolysis and Gluconeogenesis | 1 |
| Glycolysis and Gluconeogenesis, including Archaeal enzymes | 1 |
| Mannose Metabolism | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Glycolysis / Gluconeogenesis
- Fructose and mannose metabolism
- Galactose metabolism
- Pyrimidine metabolism
- Starch and sucrose metabolism
- Nucleotide sugars metabolism
- Streptomycin biosynthesis
- Aminosugars metabolism
- Drug metabolism - other enzymes
- Biosynthesis of phenylpropanoids
- Biosynthesis of terpenoids and steroids
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of plant hormones
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| pyrimidine ribonucleosides salvage I | 3 | 3 | 2 |
| trehalose degradation I (low osmolarity) | 2 | 1 | 1 |
| trehalose degradation II (cytosolic) | 2 | 1 | 1 |
| trehalose degradation IV | 3 | 2 | 1 |
| GDP-α-D-glucose biosynthesis | 3 | 2 | 1 |
| trehalose degradation V | 3 | 2 | 1 |
| sucrose degradation III (sucrose invertase) | 4 | 3 | 1 |
| superpathway of pyrimidine ribonucleosides salvage | 10 | 9 | 2 |
| glucose and glucose-1-phosphate degradation | 5 | 3 | 1 |
| glycogen degradation II | 6 | 4 | 1 |
| UDP-N-acetyl-D-glucosamine biosynthesis II | 6 | 3 | 1 |
| UDP-N-acetyl-D-galactosamine biosynthesis II | 7 | 4 | 1 |
| glycogen degradation I | 8 | 7 | 1 |
| sucrose biosynthesis II | 8 | 5 | 1 |
| 1,3-propanediol biosynthesis (engineered) | 9 | 6 | 1 |
| chitin biosynthesis | 9 | 4 | 1 |
| nucleoside and nucleotide degradation (archaea) | 10 | 4 | 1 |
| glycolysis III (from glucose) | 11 | 11 | 1 |
| homolactic fermentation | 12 | 11 | 1 |
| Bifidobacterium shunt | 15 | 12 | 1 |
| heterolactic fermentation | 18 | 14 | 1 |