Experiment set1IT088 for Caulobacter crescentus NA1000

L-Asparagine nitrogen source

Group: nitrogen source
Media: M2_noNitrogen + L-Asparagine (20 mM)
Culturing: Caulo_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 5.1 generations
By: Adam on 22-Mar-17
Media components: 1.74 g/L Disodium phosphate, 1.06 g/L Potassium phosphate monobasic, 20 mM D-Glucose, 0.5 mM Magnesium sulfate, 0.5 mM Calcium chloride, 0.01 mM Iron (II) sulfate heptahydrate, 0.008 mM EDTA
Growth plate: 1525 B2

Specific Phenotypes

For 4 genes in this experiment

For nitrogen source L-Asparagine in Caulobacter crescentus NA1000

For nitrogen source L-Asparagine across organisms

SEED Subsystems

Subsystem	#Specific
Methionine Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Nitrogen metabolism
• Alanine and aspartate metabolism
• Methionine metabolism
• Cysteine metabolism
• gamma-Hexachlorocyclohexane degradation
• Fluorobenzoate degradation
• Selenoamino acid metabolism
• Cyanoamino acid metabolism
• 1,4-Dichlorobenzene degradation
• Sulfur metabolism
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-asparagine degradation I	1	1	1
L-asparagine degradation III (mammalian)	3	3	1
superpathway of L-aspartate and L-asparagine biosynthesis	4	2	1
N-(1-deoxy-D-fructos-1-yl)-L-asparagine degradation	5	2	1
4-chlorocatechol degradation	5	2	1
3-chlorocatechol degradation I (ortho)	5	1	1
3-chlorocatechol degradation II (ortho)	5	1	1
cyanophycin metabolism	6	2	1
hydrogen sulfide biosynthesis II (mammalian)	6	1	1
4,5-dichlorocatechol degradation	7	3	1
3,5-dichlorocatechol degradation	8	3	1
3,4,6-trichlorocatechol degradation	9	4	1
1,4-dichlorobenzene degradation	9	3	1