Experiment set1IT088 for Bacteroides thetaiotaomicron VPI-5482

D-Galacturonic Acid monohydrate carbon source

Group: carbon source
Media: Varel_Bryant_medium + D-Galacturonic Acid monohydrate (20 mM)
Culturing: Btheta_ML6, 24-well transparent microplate, Anaerobic, at 37 (C), shaken=0 rpm
By: Hualan on 8-May-17
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 7 genes in this experiment

For carbon source D-Galacturonic Acid monohydrate in Bacteroides thetaiotaomicron VPI-5482

For carbon source D-Galacturonic Acid monohydrate across organisms

SEED Subsystems

Subsystem	#Specific
D-Galacturonate and D-Glucuronate Utilization	2
Alginate metabolism	1
D-Tagatose and Galactitol Utilization	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycolysis / Gluconeogenesis
• Pentose and glucuronate interconversions
• Fructose and mannose metabolism
• Galactose metabolism
• Ascorbate and aldarate metabolism
• Pyrimidine metabolism
• Starch and sucrose metabolism
• Nucleotide sugars metabolism
• Aminosugars metabolism
• Drug metabolism - other enzymes

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
pyrimidine ribonucleosides salvage I	3	3	2
4-deoxy-L-threo-hex-4-enopyranuronate degradation	5	5	1
superpathway of pyrimidine ribonucleosides salvage	10	9	2
nucleoside and nucleotide degradation (archaea)	10	4	1
superpathway of microbial D-galacturonate and D-glucuronate degradation	31	15	1