Experiment set1IT086 for Bacteroides thetaiotaomicron VPI-5482

D-Xylose carbon source

Group: carbon source
Media: Varel_Bryant_medium + D-Xylose (20 mM)
Culturing: Btheta_ML6, 24-well transparent microplate, Anaerobic, at 37 (C), shaken=0 rpm
By: Hualan on 8-May-17
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 3 genes in this experiment

For carbon source D-Xylose in Bacteroides thetaiotaomicron VPI-5482

For carbon source D-Xylose across organisms

SEED Subsystems

Subsystem	#Specific
Xylose utilization	2
Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pentose and glucuronate interconversions
• Pyrimidine metabolism
• Drug metabolism - other enzymes

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
pyrimidine ribonucleosides salvage I	3	3	2
D-xylose degradation I	2	2	1
D-arabinitol degradation I	2	1	1
xylitol degradation I	2	1	1
superpathway of pyrimidine ribonucleosides salvage	10	9	2
nucleoside and nucleotide degradation (archaea)	10	4	1
superpathway of glucose and xylose degradation	17	16	1
superpathway of pentose and pentitol degradation	42	12	1