Experiment set1IT085 for Pseudomonas fluorescens FW300-N2E3
LB with Cobalt chloride hexahydrate 0.32 mM
Group: stressMedia: LB + Cobalt chloride hexahydrate (0.32 mM), pH=7
Culturing: pseudo3_N2E3_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 28 (C), shaken=200 rpm
By: Jayashree on 4/28/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 891 B1,B2
Specific Phenotypes
For 8 genes in this experiment
For stress Cobalt chloride hexahydrate in Pseudomonas fluorescens FW300-N2E3
For stress Cobalt chloride hexahydrate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Cobalt-zinc-cadmium resistance | 1 |
| Folate Biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Tryptophan metabolism
- One carbon pool by folate
- Methane metabolism
- Folate biosynthesis
- Biosynthesis of phenylpropanoids
- Biosynthesis of alkaloids derived from terpenoid and polyketide
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| dTMP de novo biosynthesis (mitochondrial) | 3 | 3 | 1 |
| tetrahydrofolate biosynthesis I | 3 | 3 | 1 |
| tetrahydromonapterin biosynthesis | 4 | 3 | 1 |
| folate transformations III (E. coli) | 9 | 9 | 1 |
| superpathway of tetrahydrofolate biosynthesis | 10 | 8 | 1 |
| folate transformations II (plants) | 11 | 10 | 1 |
| superpathway of tetrahydrofolate biosynthesis and salvage | 12 | 10 | 1 |
| superpathway of chorismate metabolism | 59 | 43 | 1 |