Experiment set1IT081 for Phocaeicola vulgatus CL09T03C04

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D-Arabinose 20 mM carbon source

Group: carbon source
Media: Varel_Bryant_medium + D-Arabinose (20 mM)
Culturing: Bvulgatus_CL09T03C04_ML5, 96 deep-well microplate; 0.5 mL volume, Anaerobic, at 34 (C), shaken=0 rpm
By: Surya Tripathi on 12/22/21
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 5 genes in this experiment

For carbon source D-Arabinose in Phocaeicola vulgatus CL09T03C04

For carbon source D-Arabinose across organisms

SEED Subsystems

Subsystem #Specific
L-fucose utilization 2
L-fucose utilization temp 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
GDP-L-fucose biosynthesis II (from L-fucose) 2 1 1
L-fucose degradation I 4 2 1
Arg/N-end rule pathway (eukaryotic) 14 6 2
superpathway of fucose and rhamnose degradation 12 9 1