Experiment set1IT078 for Bacteroides thetaiotaomicron VPI-5482
Beta-Lactose carbon source
Group: carbon sourceMedia: Varel_Bryant_medium + Beta-Lactose (20 mM)
Culturing: Btheta_ML6, 24-well transparent microplate, Anaerobic, at 37 (C), shaken=0 rpm
By: Hualan on 8-May-17
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Specific Phenotypes
For 4 genes in this experiment
For carbon source Beta-Lactose in Bacteroides thetaiotaomicron VPI-5482
For carbon source Beta-Lactose across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Galactose metabolism
- Glycolysis / Gluconeogenesis
- Fructose and mannose metabolism
- Pyrimidine metabolism
- Starch and sucrose metabolism
- Other glycan degradation
- Nucleotide sugars metabolism
- Streptomycin biosynthesis
- Aminosugars metabolism
- Glycosaminoglycan degradation
- Sphingolipid metabolism
- Glycosphingolipid biosynthesis - ganglio series
- Drug metabolism - other enzymes
- Biosynthesis of phenylpropanoids
- Biosynthesis of terpenoids and steroids
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of plant hormones
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| lactose degradation III | 1 | 1 | 1 |
| pyrimidine ribonucleosides salvage I | 3 | 3 | 2 |
| trehalose degradation I (low osmolarity) | 2 | 1 | 1 |
| trehalose degradation II (cytosolic) | 2 | 1 | 1 |
| xyloglucan degradation II (exoglucanase) | 8 | 5 | 3 |
| trehalose degradation V | 3 | 2 | 1 |
| GDP-α-D-glucose biosynthesis | 3 | 2 | 1 |
| trehalose degradation IV | 3 | 2 | 1 |
| sucrose degradation III (sucrose invertase) | 4 | 3 | 1 |
| superpathway of pyrimidine ribonucleosides salvage | 10 | 9 | 2 |
| glucose and glucose-1-phosphate degradation | 5 | 3 | 1 |
| glycogen degradation II | 6 | 4 | 1 |
| UDP-N-acetyl-D-glucosamine biosynthesis II | 6 | 3 | 1 |
| UDP-N-acetyl-D-galactosamine biosynthesis II | 7 | 4 | 1 |
| glycogen degradation I | 8 | 7 | 1 |
| sucrose biosynthesis II | 8 | 5 | 1 |
| 1,3-propanediol biosynthesis (engineered) | 9 | 6 | 1 |
| chitin biosynthesis | 9 | 4 | 1 |
| nucleoside and nucleotide degradation (archaea) | 10 | 4 | 1 |
| glycolysis III (from glucose) | 11 | 11 | 1 |
| homolactic fermentation | 12 | 11 | 1 |
| Bifidobacterium shunt | 15 | 12 | 1 |
| heterolactic fermentation | 18 | 14 | 1 |