Experiment set1IT076 for Caulobacter crescentus NA1000

Adenosine nitrogen source

Group: nitrogen source
Media: M2_noNitrogen + Adenosine (2 mM)
Culturing: Caulo_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 5.0 generations
By: Adam on 22-Mar-17
Media components: 1.74 g/L Disodium phosphate, 1.06 g/L Potassium phosphate monobasic, 20 mM D-Glucose, 0.5 mM Magnesium sulfate, 0.5 mM Calcium chloride, 0.01 mM Iron (II) sulfate heptahydrate, 0.008 mM EDTA
Growth plate: 1526 B4

Specific Phenotypes

For 22 genes in this experiment

For nitrogen source Adenosine in Caulobacter crescentus NA1000

For nitrogen source Adenosine across organisms

SEED Subsystems

Subsystem	#Specific
Molybdenum cofactor biosynthesis	3
Methionine Biosynthesis	2
Purine Utilization	2
Chorismate: Intermediate for synthesis of PAPA antibiotics, PABA, anthranilate, 3-hydroxyanthranilate and more.	1
Folate Biosynthesis	1
Glutathione: Non-redox reactions	1
Potassium homeostasis	1
Purine conversions	1
Transport of Molybdenum	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Purine metabolism
• Methionine metabolism
• Caffeine metabolism
• Cysteine metabolism
• Valine, leucine and isoleucine degradation
• Valine, leucine and isoleucine biosynthesis
• gamma-Hexachlorocyclohexane degradation
• Fluorobenzoate degradation
• Selenoamino acid metabolism
• Glutathione metabolism
• 1,4-Dichlorobenzene degradation
• One carbon pool by folate
• Pantothenate and CoA biosynthesis
• Folate biosynthesis
• Nitrogen metabolism
• Sulfur metabolism
• Metabolism of xenobiotics by cytochrome P450
• Drug metabolism - cytochrome P450
• Biosynthesis of phenylpropanoids
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
adenosine nucleotides degradation II	5	4	3
L-alanine biosynthesis I	2	2	1
4-aminobenzoate biosynthesis I	2	2	1
4-aminobenzoate biosynthesis II	2	1	1
L-isoleucine biosynthesis V	3	2	1
L-valine degradation II	3	2	1
L-leucine degradation III	3	2	1
urate conversion to allantoin I	3	2	1
L-isoleucine degradation II	3	2	1
L-methionine salvage from L-homocysteine	3	1	1
purine deoxyribonucleosides degradation II	3	1	1
L-isoleucine degradation III (oxidative Stickland reaction)	3	1	1
adenine and adenosine salvage V	3	1	1
L-valine degradation III (oxidative Stickland reaction)	3	1	1
L-leucine degradation V (oxidative Stickland reaction)	3	1	1
ureide biosynthesis	7	5	2
purine nucleotides degradation II (aerobic)	11	8	3
L-valine biosynthesis	4	4	1
superpathway of L-alanine biosynthesis	4	4	1
L-methionine biosynthesis III	4	4	1
guanosine nucleotides degradation II	4	4	1
adenosine nucleotides degradation I	8	7	2
guanosine nucleotides degradation III	4	3	1
guanosine nucleotides degradation I	4	3	1
inosine 5'-phosphate degradation	4	3	1
adenine and adenosine salvage III	4	2	1
purine deoxyribonucleosides degradation I	4	1	1
4-hydroxy-2-nonenal detoxification	4	1	1
L-methionine biosynthesis I	5	3	1
4-chlorocatechol degradation	5	2	1
pentachlorophenol degradation	10	3	2
3-chlorocatechol degradation I (ortho)	5	1	1
3-chlorocatechol degradation II (ortho)	5	1	1
L-leucine degradation IV (reductive Stickland reaction)	5	1	1
superpathway of branched chain amino acid biosynthesis	17	17	3
L-leucine biosynthesis	6	6	1
purine nucleotides degradation I (plants)	12	10	2
superpathway of guanosine nucleotides degradation (plants)	6	5	1
L-leucine degradation I	6	5	1
superpathway of purines degradation in plants	18	12	3
L-isoleucine biosynthesis IV	6	4	1
L-isoleucine degradation I	6	4	1
purine nucleobases degradation II (anaerobic)	24	14	4
molybdopterin biosynthesis	6	3	1
purine ribonucleosides degradation	6	1	1
hydrogen sulfide biosynthesis II (mammalian)	6	1	1
L-isoleucine biosynthesis I (from threonine)	7	7	1
L-isoleucine biosynthesis III	7	4	1
4,5-dichlorocatechol degradation	7	3	1
caffeine degradation III (bacteria, via demethylation)	7	1	1
superpathway of purine deoxyribonucleosides degradation	7	1	1
L-isoleucine biosynthesis II	8	6	1
superpathway of L-homoserine and L-methionine biosynthesis	8	6	1
L-valine degradation I	8	5	1
3,5-dichlorocatechol degradation	8	3	1
glutathione-mediated detoxification I	8	3	1
folate transformations III (E. coli)	9	8	1
superpathway of S-adenosyl-L-methionine biosynthesis	9	7	1
superpathway of L-methionine biosynthesis (transsulfuration)	9	7	1
3,4,6-trichlorocatechol degradation	9	4	1
1,4-dichlorobenzene degradation	9	3	1
gliotoxin biosynthesis	9	2	1
theophylline degradation	9	1	1
glutathione-mediated detoxification II	9	1	1
superpathway of tetrahydrofolate biosynthesis	10	8	1
caffeine degradation IV (bacteria, via demethylation and oxidation)	10	1	1
folate transformations II (plants)	11	8	1
superpathway of candicidin biosynthesis	11	4	1
superpathway of L-methionine biosynthesis (by sulfhydrylation)	12	12	1
superpathway of tetrahydrofolate biosynthesis and salvage	12	10	1
indole glucosinolate activation (intact plant cell)	12	3	1
camalexin biosynthesis	12	2	1
superpathway of L-isoleucine biosynthesis I	13	13	1
folate transformations I	13	8	1
superpathway of L-methionine salvage and degradation	16	7	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I	18	16	1
superpathway of L-threonine metabolism	18	9	1
aspartate superpathway	25	23	1
anteiso-branched-chain fatty acid biosynthesis	34	24	1
even iso-branched-chain fatty acid biosynthesis	34	24	1
odd iso-branched-chain fatty acid biosynthesis	34	24	1
superpathway of chorismate metabolism	59	38	1