Experiment set1IT075 for Bacteroides thetaiotaomicron VPI-5482

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D-Raffinose pentahydrate carbon source

Group: carbon source
Media: Varel_Bryant_medium + D-Raffinose pentahydrate (20 mM)
Culturing: Btheta_ML6, 24-well transparent microplate, Anaerobic, at 37 (C), shaken=0 rpm
By: Hualan on 8-May-17
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 4 genes in this experiment

For carbon source D-Raffinose pentahydrate in Bacteroides thetaiotaomicron VPI-5482

For carbon source D-Raffinose pentahydrate across organisms

SEED Subsystems

Subsystem #Specific
Entner-Doudoroff Pathway 1
Glycolysis and Gluconeogenesis 1
Glycolysis and Gluconeogenesis, including Archaeal enzymes 1
Inositol catabolism 1
Mannose Metabolism 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
pyrimidine ribonucleosides salvage I 3 3 2
trehalose degradation I (low osmolarity) 2 1 1
trehalose degradation II (cytosolic) 2 1 1
GDP-α-D-glucose biosynthesis 3 2 1
trehalose degradation V 3 2 1
trehalose degradation IV 3 2 1
sucrose degradation III (sucrose invertase) 4 3 1
superpathway of pyrimidine ribonucleosides salvage 10 9 2
glucose and glucose-1-phosphate degradation 5 3 1
myo-inositol degradation II 5 1 1
glycogen degradation II 6 4 1
UDP-N-acetyl-D-glucosamine biosynthesis II 6 3 1
UDP-N-acetyl-D-galactosamine biosynthesis II 7 4 1
myo-inositol degradation I 7 1 1
glycogen degradation I 8 7 1
sucrose biosynthesis II 8 5 1
1,3-propanediol biosynthesis (engineered) 9 6 1
chitin biosynthesis 9 4 1
nucleoside and nucleotide degradation (archaea) 10 4 1
myo-, chiro- and scyllo-inositol degradation 10 1 1
glycolysis III (from glucose) 11 11 1
homolactic fermentation 12 11 1
Bifidobacterium shunt 15 12 1
heterolactic fermentation 18 14 1
streptomycin biosynthesis 18 3 1