Experiment set1IT071 for Pantoea sp. MT58

Potassium dichromate 5 uM, aerobic

Group: stress
Media: DefinedMedia_AdamsLab_Glucose + Potassium dichromate (5 uM), pH=7
Culturing: MT058_ML2, hungate, Aerobic, at 24 (C), shaken=150 rpm
Growth: about 5.5 generations
By: Mike Thorgersen on 19-Feb-19
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 5 mM Sodium phosphate monobasic monohydrate, 2.5 g/L Sodium bicarbonate, 20 mM D-Glucose, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 10 genes in this experiment

For stress Potassium dichromate in Pantoea sp. MT58

For stress Potassium dichromate across organisms

SEED Subsystems

Subsystem	#Specific
Cysteine Biosynthesis	1
Methionine Biosynthesis	1
YeiH	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Cysteine metabolism
• Phenylalanine, tyrosine and tryptophan biosynthesis
• Selenoamino acid metabolism
• Sulfur metabolism
• Biosynthesis of phenylpropanoids
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-cysteine biosynthesis I	2	2	1
L-cysteine biosynthesis VII (from S-sulfo-L-cysteine)	4	3	1
seleno-amino acid biosynthesis (plants)	5	3	1
L-tryptophan biosynthesis	6	6	1
hydrogen sulfide biosynthesis II (mammalian)	6	1	1
superpathway of sulfate assimilation and cysteine biosynthesis	9	9	1
superpathway of L-tryptophan biosynthesis	13	13	1
superpathway of aromatic amino acid biosynthesis	18	18	1
superpathway of seleno-compound metabolism	19	8	1
superpathway of chorismate metabolism	59	44	1