Experiment set1IT070 for Caulobacter crescentus NA1000

L-Tryptophan nitrogen source

Group: nitrogen source
Media: M2_noNitrogen + L-Tryptophan (5 mM)
Culturing: Caulo_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 4.3 generations
By: Adam on 22-Mar-17
Media components: 1.74 g/L Disodium phosphate, 1.06 g/L Potassium phosphate monobasic, 20 mM D-Glucose, 0.5 mM Magnesium sulfate, 0.5 mM Calcium chloride, 0.01 mM Iron (II) sulfate heptahydrate, 0.008 mM EDTA
Growth plate: 1524 D2

Specific Phenotypes

For 9 genes in this experiment

For nitrogen source L-Tryptophan in Caulobacter crescentus NA1000

For nitrogen source L-Tryptophan across organisms

SEED Subsystems

Subsystem	#Specific
Aromatic amino acid degradation	2
Pyridoxin (Vitamin B6) Biosynthesis	1
Terminal cytochrome C oxidases	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Tryptophan metabolism
• Oxidative phosphorylation
• Urea cycle and metabolism of amino groups
• Glycine, serine and threonine metabolism
• Arginine and proline metabolism
• Histidine metabolism
• Tyrosine metabolism
• Phenylalanine metabolism
• Vitamin B6 metabolism
• Alkaloid biosynthesis I
• Drug metabolism - cytochrome P450
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
indole-3-acetate biosynthesis III (bacteria)	2	2	1
pyridoxal 5'-phosphate salvage I	6	5	3
arsenite to oxygen electron transfer	2	1	1
phenylethylamine degradation I	2	1	1
pyridoxal 5'-phosphate salvage II (plants)	9	8	3
L-tryptophan degradation VI (via tryptamine)	3	1	1
arsenite to oxygen electron transfer (via azurin)	3	1	1
noradrenaline and adrenaline degradation	13	8	4
superpathway of pyridoxal 5'-phosphate biosynthesis and salvage	12	10	3
aerobic respiration I (cytochrome c)	4	3	1
4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis II	8	4	2
aerobic respiration II (cytochrome c) (yeast)	4	2	1
salidroside biosynthesis	4	2	1
putrescine degradation III	4	2	1
L-tryptophan degradation X (mammalian, via tryptamine)	4	2	1
aromatic biogenic amine degradation (bacteria)	8	3	2
melatonin degradation II	4	1	1
phenylethanol biosynthesis	5	2	1
dopamine degradation	5	2	1
Fe(II) oxidation	6	3	1
serotonin degradation	7	4	1
L-phenylalanine degradation IV (mammalian, via side chain)	9	3	1
superpathway of phenylethylamine degradation	11	5	1
superpathway of melatonin degradation	11	1	1