Experiment set1IT069 for Pseudomonas fluorescens FW300-N2E3
D-Fructose carbon source
Group: carbon sourceMedia: RCH2_defined_noCarbon + D-Fructose (20 mM), pH=7
Culturing: pseudo3_N2E3_ML2, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 6.9 generations
By: Jayashree on 4/28/2014
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Specific Phenotypes
For 6 genes in this experiment
For carbon source D-Fructose in Pseudomonas fluorescens FW300-N2E3
For carbon source D-Fructose across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Fructose utilization | 2 |
| Mannitol Utilization | 2 |
| Fructose and Mannose Inducible PTS | 1 |
| Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Fructose and mannose metabolism
- Nucleotide sugars metabolism
- Glycolysis / Gluconeogenesis
- Galactose metabolism
- Ascorbate and aldarate metabolism
- Starch and sucrose metabolism
- Aminosugars metabolism
- Pentose phosphate pathway
- C21-Steroid hormone metabolism
- Glycine, serine and threonine metabolism
- Bisphenol A degradation
- Streptomycin biosynthesis
- Polyketide sugar unit biosynthesis
- Linoleic acid metabolism
- Tetrachloroethene degradation
- Benzoate degradation via CoA ligation
- Butanoate metabolism
- Retinol metabolism
- Alkaloid biosynthesis I
- Insect hormone biosynthesis
- Biosynthesis of unsaturated fatty acids
- Biosynthesis of type II polyketide products
- Biosynthesis of alkaloids derived from shikimate pathway
MetaCyc Pathways
Pathways that contain genes with specific phenotypes: