Experiment set1IT069 for Azospirillum brasilense Sp245
a-Ketoglutaric acid disodium salt hydrate carbon source
Group: carbon sourceMedia: RCH2_defined_noCarbon + a-Ketoglutaric acid disodium salt hydrate (20 mM), pH=7
Culturing: AzoBra_ML2a, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
By: Mark on 3/5/2015
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Specific Phenotypes
For 7 genes in this experiment
For carbon source a-Ketoglutaric acid disodium salt hydrate in Azospirillum brasilense Sp245
For carbon source a-Ketoglutaric acid disodium salt hydrate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Bacterial Chemotaxis | 1 |
| HMG CoA Synthesis | 1 |
| Leucine Degradation and HMG-CoA Metabolism | 1 |
| Oxidative stress | 1 |
| Oxygen and light sensor PpaA-PpsR | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| stearate biosynthesis I (animals) | 6 | 1 | 1 |
| 2-deoxy-D-ribose degradation II | 8 | 3 | 1 |
| sphingosine and sphingosine-1-phosphate metabolism | 10 | 4 | 1 |
| ceramide and sphingolipid recycling and degradation (yeast) | 16 | 4 | 1 |
| palmitate biosynthesis III | 29 | 28 | 1 |
| palmitate biosynthesis II (type II fatty acid synthase) | 31 | 29 | 1 |
| superpathway of fatty acid biosynthesis II (plant) | 43 | 38 | 1 |
| superpathway of fatty acids biosynthesis (E. coli) | 53 | 48 | 1 |