Experiment set1IT064 for Acidovorax sp. GW101-3H11

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L-Arabinose carbon source

Group: carbon source
Media: RCH2_defined_noCarbon + L-Arabinose (20 mM), pH=7
Culturing: acidovorax_3H11_ML3a, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
By: Mark on 3/3/2015
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 6 genes in this experiment

For carbon source L-Arabinose in Acidovorax sp. GW101-3H11

For carbon source L-Arabinose across organisms

SEED Subsystems

Subsystem #Specific
L-Arabinose utilization 4
D-ribose utilization 1
NAD and NADP cofactor biosynthesis global 1
NAD regulation 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
D-galactose degradation II 2 2 2
NAD salvage pathway II (PNC IV cycle) 5 4 2
NAD de novo biosynthesis I 6 5 2
NAD de novo biosynthesis III 6 4 2
NAD de novo biosynthesis IV (anaerobic) 6 4 2
NAD salvage pathway I (PNC VI cycle) 7 5 2
NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde 4 3 1
NAD salvage pathway V (PNC V cycle) 5 3 1
L-arabinose degradation III 6 5 1
L-arabinose degradation V 6 3 1
L-arabinose degradation IV 8 5 1
NAD de novo biosynthesis II (from tryptophan) 9 6 1
NAD salvage (plants) 11 5 1
aspartate superpathway 25 22 2
superpathway of NAD biosynthesis in eukaryotes 14 9 1
superpathway of pentose and pentitol degradation 42 14 1