Experiment set1IT060 for Pantoea sp. MT58

Cadmium acetate dihydrate 8 uM, aerobic

Group: stress
Media: DefinedMedia_AdamsLab_Glucose + Cadmium acetate dihydrate (8 uM), pH=7
Culturing: MT058_ML2, hungate, Aerobic, at 24 (C), shaken=150 rpm
Growth: about 5.7 generations
By: Mike Thorgersen on 19-Feb-19
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 5 mM Sodium phosphate monobasic monohydrate, 2.5 g/L Sodium bicarbonate, 20 mM D-Glucose, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 2 genes in this experiment

For stress Cadmium acetate dihydrate in Pantoea sp. MT58

For stress Cadmium acetate dihydrate across organisms

SEED Subsystems

Subsystem	#Specific
Coenzyme A Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pantothenate and CoA biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
phosphopantothenate biosynthesis I	4	4	1
phosphopantothenate biosynthesis III (archaea)	4	2	1
superpathway of coenzyme A biosynthesis I (bacteria)	9	9	1
superpathway of coenzyme A biosynthesis II (plants)	10	5	1