Experiment set1IT059 for Caulobacter crescentus NA1000
L-Glutamic acid monopotassium salt monohydrate nitrogen source
Group: nitrogen sourceMedia: M2_noNitrogen + L-Glutamic acid monopotassium salt monohydrate (20 mM)
Culturing: Caulo_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 5.5 generations
By: Adam on 22-Mar-17
Media components: 1.74 g/L Disodium phosphate, 1.06 g/L Potassium phosphate monobasic, 20 mM D-Glucose, 0.5 mM Magnesium sulfate, 0.5 mM Calcium chloride, 0.01 mM Iron (II) sulfate heptahydrate, 0.008 mM EDTA
Growth plate: 1524 A5
Specific Phenotypes
For 7 genes in this experiment
For nitrogen source L-Glutamic acid monopotassium salt monohydrate in Caulobacter crescentus NA1000
For nitrogen source L-Glutamic acid monopotassium salt monohydrate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Glutathione: Non-redox reactions | 1 |
| Potassium homeostasis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- gamma-Hexachlorocyclohexane degradation
- Fluorobenzoate degradation
- Glutathione metabolism
- 1,4-Dichlorobenzene degradation
- Metabolism of xenobiotics by cytochrome P450
- Drug metabolism - cytochrome P450
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| 4-hydroxy-2-nonenal detoxification | 4 | 1 | 1 |
| 4-chlorocatechol degradation | 5 | 2 | 1 |
| pentachlorophenol degradation | 10 | 3 | 2 |
| 3-chlorocatechol degradation I (ortho) | 5 | 1 | 1 |
| 3-chlorocatechol degradation II (ortho) | 5 | 1 | 1 |
| 4,5-dichlorocatechol degradation | 7 | 3 | 1 |
| glutathione-mediated detoxification I | 8 | 3 | 1 |
| 3,5-dichlorocatechol degradation | 8 | 3 | 1 |
| 3,4,6-trichlorocatechol degradation | 9 | 4 | 1 |
| 1,4-dichlorobenzene degradation | 9 | 3 | 1 |
| gliotoxin biosynthesis | 9 | 2 | 1 |
| glutathione-mediated detoxification II | 9 | 1 | 1 |
| indole glucosinolate activation (intact plant cell) | 12 | 3 | 1 |
| camalexin biosynthesis | 12 | 2 | 1 |