Experiment set1IT059 for Phocaeicola vulgatus CL09T03C04

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Sucrose 20 mM carbon source

Group: carbon source
Media: Varel_Bryant_medium + Sucrose (20 mM)
Culturing: Bvulgatus_CL09T03C04_ML5, 96 deep-well microplate; 0.5 mL volume, Anaerobic, at 34 (C), shaken=0 rpm
By: Surya Tripathi on 12/22/21
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 9 genes in this experiment

For carbon source Sucrose in Phocaeicola vulgatus CL09T03C04

For carbon source Sucrose across organisms

SEED Subsystems

Subsystem #Specific
CMP-N-acetylneuraminate Biosynthesis 1
Entner-Doudoroff Pathway 1
Pentose phosphate pathway 1
Sialic Acid Metabolism 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
UDP-N-acetyl-α-D-mannosamine biosynthesis 1 1 1
pentose phosphate pathway (oxidative branch) I 3 3 1
CMP-N-acetylneuraminate biosynthesis II (bacteria) 3 2 1
CMP-N-acetylneuraminate biosynthesis I (eukaryotes) 5 2 1
formaldehyde oxidation I 6 3 1
pentose phosphate pathway 8 8 1
Entner-Doudoroff pathway I 9 8 1
superpathway of CMP-sialic acids biosynthesis 15 3 1
superpathway of glucose and xylose degradation 17 16 1
superpathway of glycolysis and the Entner-Doudoroff pathway 17 14 1
heterolactic fermentation 18 14 1
superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis 24 4 1