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Experiment set1IT056 for
Methanococcus maripaludis JJ
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Formate pooled triplicate planktonic cells
200 most detrimental genes:
gene
name
fitness
t score
description
MMJJ
_RS08870
+0.9
1.1
2-isopropylmalate synthase
compare
MMJJ
_RS01210
+0.7
1.7
hypothetical protein
compare
MMJJ
_RS06275
+0.5
1.7
hypothetical protein
compare
MMJJ
_RS09325
+0.5
2.7
hypothetical protein
compare
MMJJ
_RS06540
+0.5
1.8
DUF2120 domain-containing protein
compare
MMJJ
_RS06770
+0.5
4.3
hypothetical protein
compare
MMJJ
_RS06710
+0.5
4.1
TldD/PmbA family protein
compare
MMJJ
_RS06950
+0.5
3.2
4-demethylwyosine synthase TYW1
compare
MMJJ
_RS06470
+0.5
3.1
tripartite tricarboxylate transporter permease
compare
MMJJ
_RS07225
+0.5
3.1
coenzyme F420 hydrogenase subunit gamma
compare
MMJJ
_RS07610
+0.5
2.3
DUF2119 domain-containing protein
compare
MMJJ
_RS06475
+0.4
2.7
AAA family ATPase
compare
MMJJ
_RS06135
+0.4
3.2
50S ribosomal protein L11 methyltransferase
compare
MMJJ
_RS07205
+0.4
1.4
MTH895/ArsE family thioredoxin-like protein
compare
MMJJ
_RS07270
+0.4
2.8
ribonuclease HII
compare
MMJJ
_RS06460
+0.4
2.4
cytochrome c
compare
MMJJ
_RS06800
+0.4
2.2
prefoldin subunit alpha
compare
MMJJ
_RS06585
+0.4
2.7
alanine racemase
compare
MMJJ
_RS05115
+0.4
1.8
P-II family nitrogen regulator
compare
MMJJ
_RS07210
+0.4
2.9
AmmeMemoRadiSam system protein B
compare
MMJJ
_RS07420
+0.4
3.1
protease complex subunit PrcB family protein
compare
MMJJ
_RS07015
+0.4
0.5
PFL family protein
compare
MMJJ
_RS06280
+0.4
2.9
hypothetical protein
compare
MMJJ
_RS05510
+0.4
3.0
archaellum operon transcriptional activator EarA family protein
compare
MMJJ
_RS07690
+0.4
2.5
glycosyltransferase family 4 protein
compare
MMJJ
_RS06130
+0.4
2.4
hypothetical protein
compare
MMJJ
_RS07620
+0.4
2.7
class I SAM-dependent rRNA methyltransferase
compare
MMJJ
_RS07255
+0.4
3.4
hypothetical protein
compare
MMJJ
_RS06665
+0.4
1.6
DUF3236 domain-containing protein
compare
MMJJ
_RS06810
+0.4
2.9
L-serine ammonia-lyase, iron-sulfur-dependent, subunit alpha
compare
MMJJ
_RS06105
+0.4
2.7
DUF99 family protein
compare
MMJJ
_RS07215
+0.4
3.3
TIGR00341 family protein
compare
MMJJ
_RS02905
+0.4
1.3
NifB/NifX family molybdenum-iron cluster-binding protein
compare
MMJJ
_RS04375
+0.4
1.8
hypothetical protein
compare
MMJJ
_RS06630
+0.4
2.7
4Fe-4S dicluster domain-containing protein
compare
MMJJ
_RS06515
+0.4
2.8
ribonuclease III
compare
MMJJ
_RS07765
+0.4
1.7
DMT family protein
compare
MMJJ
_RS07200
+0.4
3.0
MFS transporter
compare
MMJJ
_RS07240
+0.4
3.1
MBL fold metallo-hydrolase
compare
MMJJ
_RS06450
+0.4
2.7
DUF3343 domain-containing protein
compare
MMJJ
_RS06765
+0.4
3.0
hypothetical protein
compare
MMJJ
_RS06550
+0.4
2.6
ABC transporter permease
compare
MMJJ
_RS07385
+0.3
2.5
ribosomal protein S18-alanine N-acetyltransferase
compare
MMJJ
_RS01615
+0.3
1.0
MoaD/ThiS family protein
compare
MMJJ
_RS06720
+0.3
2.5
mechanosensitive ion channel family protein
compare
MMJJ
_RS05505
+0.3
3.1
hypothetical protein
compare
MMJJ
_RS06075
+0.3
2.3
DNA-binding protein Alba
compare
MMJJ
_RS06445
+0.3
2.7
hypothetical protein
compare
MMJJ
_RS06685
+0.3
2.8
S-layer protein
compare
MMJJ
_RS07965
+0.3
1.2
hypothetical protein
compare
MMJJ
_RS04525
+0.3
1.1
hypothetical protein
compare
MMJJ
_RS01060
+0.3
1.2
ABC transporter ATP-binding protein
compare
MMJJ
_RS06465
+0.3
2.0
hypothetical protein
compare
MMJJ
_RS06495
+0.3
1.1
hypothetical protein
compare
MMJJ
_RS06960
+0.3
2.8
CAP domain-containing protein
compare
MMJJ
_RS06120
+0.3
1.9
DUF4013 domain-containing protein
compare
MMJJ
_RS06575
+0.3
0.4
prephenate dehydrogenase
compare
MMJJ
_RS08165
+0.3
1.5
thermonuclease family protein
compare
MMJJ
_RS00305
+0.3
1.9
chemotaxis protein CheC
compare
MMJJ
_RS07685
+0.3
2.5
glycogen/starch synthase
compare
MMJJ
_RS06205
+0.3
2.1
EamA family transporter
compare
MMJJ
_RS05980
+0.3
1.9
DUF5400 domain-containing protein
compare
MMJJ
_RS02285
+0.3
1.5
class III signal peptide-containing protein
compare
MMJJ
_RS06555
+0.3
2.5
ATP-binding cassette domain-containing protein
compare
MMJJ
_RS06640
+0.3
2.0
YfcE family phosphodiesterase
compare
MMJJ
_RS06430
+0.3
1.8
haloacid dehalogenase
compare
MMJJ
_RS06580
+0.3
2.3
alanine dehydrogenase
compare
MMJJ
_RS07825
+0.3
1.8
YciI family protein
compare
MMJJ
_RS07230
+0.3
1.8
coenzyme F420-reducing hydrogenase, FrhD protein
compare
MMJJ
_RS07365
+0.3
2.2
CatB-related O-acetyltransferase
compare
MMJJ
_RS07495
+0.3
2.2
4Fe-4S binding protein
compare
MMJJ
_RS06055
+0.3
2.1
S-methyl-5-thioribose-1-phosphate isomerase
compare
MMJJ
_RS07235
+0.3
2.3
coenzyme F420 hydrogenase subunit alpha
compare
MMJJ
_RS07450
+0.3
1.5
hypothetical protein
compare
MMJJ
_RS05400
+0.3
1.0
CoA-binding protein
compare
MMJJ
_RS03955
+0.3
1.7
hypothetical protein
compare
MMJJ
_RS07695
+0.3
2.3
glycoside hydrolase family 15 protein
compare
MMJJ
_RS07445
+0.3
2.1
RlmF-related methyltransferase
compare
MMJJ
_RS08310
+0.3
1.5
ZIP family metal transporter
compare
MMJJ
_RS08390
+0.3
1.2
carboxymuconolactone decarboxylase family protein
compare
MMJJ
_RS01970
+0.3
1.6
hypothetical protein
compare
MMJJ
_RS08380
+0.3
1.0
hypothetical protein
compare
MMJJ
_RS07500
+0.3
1.8
hypothetical protein
compare
MMJJ
_RS04585
+0.3
1.8
phenylacetate--CoA ligase
compare
MMJJ
_RS02365
+0.3
0.9
DUF2540 domain-containing protein
compare
MMJJ
_RS06455
+0.3
1.8
YeeE/YedE family protein
compare
MMJJ
_RS06790
+0.3
2.2
nucleotidyltransferase family protein
compare
MMJJ
_RS06520
+0.3
2.0
TldD/PmbA family protein
compare
MMJJ
_RS08630
+0.2
1.4
signal recognition particle subunit SRP19/SEC65 family protein
compare
MMJJ
_RS06125
+0.2
1.9
methanogenesis marker 16 metalloprotein
compare
MMJJ
_RS08385
+0.2
1.6
class II SORL domain-containing protein
compare
MMJJ
_RS04120
+0.2
1.3
amino acid ABC transporter ATP-binding protein
compare
MMJJ
_RS05985
+0.2
1.7
ABC transporter substrate-binding protein
compare
MMJJ
_RS08350
+0.2
2.0
heavy metal translocating P-type ATPase
compare
MMJJ
_RS06235
+0.2
2.1
IGHMBP2 family helicase
compare
MMJJ
_RS06680
+0.2
1.5
DUF366 family protein
compare
MMJJ
_RS09360
+0.2
1.7
hypothetical protein
compare
MMJJ
_RS07700
+0.2
1.9
glycoside hydrolase family 57 protein
compare
MMJJ
_RS02075
+0.2
0.9
acyl-CoA thioesterase
compare
MMJJ
_RS07390
+0.2
1.9
TIGR04014 family B12-binding domain/radical SAM domain-containing protein
compare
MMJJ
_RS07220
+0.2
1.7
coenzyme F420 hydrogenase subunit beta
compare
MMJJ
_RS00715
+0.2
1.3
DUF1847 domain-containing protein
compare
MMJJ
_RS07260
+0.2
1.7
PspC domain-containing protein
compare
MMJJ
_RS06190
+0.2
1.7
7-cyano-7-deazaguanine synthase
compare
MMJJ
_RS06340
+0.2
0.5
coenzyme-B sulfoethylthiotransferase subunit alpha
compare
MMJJ
_RS07480
+0.2
1.8
FAD-dependent oxidoreductase
compare
MMJJ
_RS09010
+0.2
1.4
23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH
compare
MMJJ
_RS07800
+0.2
1.8
AMP-binding protein
compare
MMJJ
_RS09355
+0.2
1.5
hypothetical protein
compare
MMJJ
_RS04350
+0.2
0.9
hypothetical protein
compare
MMJJ
_RS04190
+0.2
1.5
hypothetical protein
compare
MMJJ
_RS09225
+0.2
1.0
hypothetical protein
compare
MMJJ
_RS03380
+0.2
1.0
hypothetical protein
compare
MMJJ
_RS08195
+0.2
1.2
DUF4186 domain-containing protein
compare
MMJJ
_RS02950
+0.2
1.5
epoxyqueuosine reductase
compare
MMJJ
_RS06080
+0.2
1.5
serine/threonine protein kinase
compare
MMJJ
_RS03510
+0.2
1.3
acyl-CoA dehydratase activase
compare
MMJJ
_RS07025
+0.2
1.1
tRNA (cytidine(56)-2'-O)-methyltransferase
compare
MMJJ
_RS03575
+0.2
1.0
NUDIX hydrolase
compare
MMJJ
_RS08930
+0.2
1.3
5'/3'-nucleotidase SurE
compare
MMJJ
_RS02875
+0.2
1.5
site-2 protease family protein
compare
MMJJ
_RS06440
+0.2
1.2
DUF4870 domain-containing protein
compare
MMJJ
_RS08395
+0.2
1.6
CoB--CoM heterodisulfide reductase subunit B
compare
MMJJ
_RS01260
+0.2
1.5
hypothetical protein
compare
MMJJ
_RS06500
+0.2
1.4
class I SAM-dependent methyltransferase
compare
MMJJ
_RS03450
+0.2
1.3
hypothetical protein
compare
MMJJ
_RS08400
+0.2
0.8
CoB--CoM heterodisulfide reductase subunit C
compare
MMJJ
_RS06115
+0.2
1.4
pyruvate kinase
compare
MMJJ
_RS07795
+0.2
1.4
cupin domain-containing protein
compare
MMJJ
_RS09020
+0.2
0.9
hypothetical protein
compare
MMJJ
_RS05700
+0.2
1.5
metallophosphoesterase
compare
MMJJ
_RS05965
+0.2
1.6
MFS transporter
compare
MMJJ
_RS09205
+0.2
0.8
hypothetical protein
compare
MMJJ
_RS05105
+0.2
0.9
P-II family nitrogen regulator
compare
MMJJ
_RS05875
+0.2
1.7
hypothetical protein
compare
MMJJ
_RS06140
+0.2
1.5
RimK family alpha-L-glutamate ligase
compare
MMJJ
_RS05525
+0.2
1.0
GTP-binding protein
compare
MMJJ
_RS04100
+0.2
1.4
class III signal peptide-containing protein
compare
MMJJ
_RS00135
+0.2
1.2
FmdE family protein
compare
MMJJ
_RS02185
+0.2
1.0
TIGR00296 family protein
compare
MMJJ
_RS04530
+0.2
1.4
iron export ABC transporter permease subunit FetB
compare
MMJJ
_RS07855
+0.2
1.3
YkgJ family cysteine cluster protein
compare
MMJJ
_RS08735
+0.2
1.5
hypothetical protein
compare
MMJJ
_RS01035
+0.2
1.0
hypothetical protein
compare
MMJJ
_RS00140
+0.2
0.7
hypothetical protein
compare
MMJJ
_RS05095
+0.2
1.5
ammonium transporter
compare
MMJJ
_RS01425
+0.2
1.3
cell wall-binding protein
compare
MMJJ
_RS02355
+0.2
1.3
DUF2798 domain-containing protein
compare
MMJJ
_RS03770
+0.2
1.4
hypothetical protein
compare
MMJJ
_RS06160
+0.2
1.4
stage II sporulation protein M
compare
MMJJ
_RS08365
+0.2
0.6
DsrE family protein
compare
MMJJ
_RS07010
+0.2
1.0
preprotein translocase subunit Sec61beta
compare
MMJJ
_RS06285
+0.2
1.1
YchF/TatD family DNA exonuclease
compare
MMJJ
_RS01510
+0.2
1.1
ATP-binding protein
compare
MMJJ
_RS03220
+0.2
1.3
inosine/xanthosine triphosphatase
compare
MMJJ
_RS08635
+0.2
1.1
hypothetical protein
compare
MMJJ
_RS04780
+0.2
0.7
nucleic acid-binding protein
compare
MMJJ
_RS02140
+0.2
0.8
preflagellin peptidase FlaK
compare
MMJJ
_RS04810
+0.2
0.9
nitroreductase family protein
compare
MMJJ
_RS05990
+0.2
1.1
phosphatase PAP2 family protein
compare
MMJJ
_RS00555
+0.2
1.0
hypothetical protein
compare
MMJJ
_RS08490
+0.2
0.7
rubrerythrin family protein
compare
MMJJ
_RS08885
+0.2
1.2
cysteine--tRNA ligase
compare
MMJJ
_RS08745
+0.2
1.2
glycosyltransferase family 9 protein
compare
MMJJ
_RS09065
+0.2
0.8
hypothetical protein
compare
MMJJ
_RS07885
+0.2
1.2
NERD domain-containing protein
compare
MMJJ
_RS09245
+0.2
0.7
hypothetical protein
compare
MMJJ
_RS04720
+0.2
0.2
IMP dehydrogenase
compare
MMJJ
_RS04855
+0.2
1.1
ECF transporter S component
compare
MMJJ
_RS05895
+0.1
1.2
ABC transporter permease
compare
MMJJ
_RS08050
+0.1
1.2
ABC transporter substrate-binding protein
compare
MMJJ
_RS06085
+0.1
0.7
proteasome assembly chaperone family protein
compare
MMJJ
_RS08375
+0.1
1.1
ferritin
compare
MMJJ
_RS05390
+0.1
1.1
tRNA pseudouridine(13) synthase TruD
compare
MMJJ
_RS08455
+0.1
0.7
tetratricopeptide repeat protein
compare
MMJJ
_RS05890
+0.1
1.1
molybdate ABC transporter substrate-binding protein
compare
MMJJ
_RS05880
+0.1
1.3
hypothetical protein
compare
MMJJ
_RS08345
+0.1
1.2
flavodoxin family protein
compare
MMJJ
_RS07630
+0.1
1.1
hypothetical protein
compare
MMJJ
_RS01310
+0.1
1.1
phenylacetate--CoA ligase
compare
MMJJ
_RS01250
+0.1
0.7
hypothetical protein
compare
MMJJ
_RS05100
+0.1
1.0
P-II family nitrogen regulator
compare
MMJJ
_RS01505
+0.1
0.9
DUF2124 domain-containing protein
compare
MMJJ
_RS00885
+0.1
0.5
coenzyme F420-reducing hydrogenase, FrhD protein
compare
MMJJ
_RS08215
+0.1
1.0
radical SAM protein
compare
MMJJ
_RS05975
+0.1
1.0
sulfurtransferase TusA family protein
compare
MMJJ
_RS07740
+0.1
1.0
DUF2334 domain-containing protein
compare
MMJJ
_RS05200
+0.1
1.1
TIGR04013 family B12-binding domain/radical SAM domain-containing protein
compare
MMJJ
_RS05350
+0.1
0.9
DUF192 domain-containing protein
compare
MMJJ
_RS07645
+0.1
0.9
selenium-binding protein
compare
MMJJ
_RS01280
+0.1
1.0
hydroxylamine reductase
compare
MMJJ
_RS04295
+0.1
1.1
DUF2202 domain-containing protein
compare
MMJJ
_RS08045
+0.1
1.0
transporter substrate-binding domain-containing protein
compare
MMJJ
_RS04560
+0.1
0.9
ABC transporter permease
compare
MMJJ
_RS05955
+0.1
1.0
radical SAM protein
compare
MMJJ
_RS06100
+0.1
1.1
rubrerythrin
compare
MMJJ
_RS04070
+0.1
1.1
DUF2341 domain-containing protein
compare
MMJJ
_RS09145
+0.1
0.8
exodeoxyribonuclease III
compare
MMJJ
_RS05695
+0.1
0.5
class III signal peptide-containing protein
compare
MMJJ
_RS04945
+0.1
0.8
PLP-dependent aminotransferase family protein
compare
Specific Phenotypes
For 2 genes in this experiment