Experiment set1IT056 for Caulobacter crescentus NA1000

L-Arginine nitrogen source

Group: nitrogen source
Media: M2_noNitrogen + L-Arginine (10 mM)
Culturing: Caulo_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 5.3 generations
By: Adam on 22-Mar-17
Media components: 1.74 g/L Disodium phosphate, 1.06 g/L Potassium phosphate monobasic, 20 mM D-Glucose, 0.5 mM Magnesium sulfate, 0.5 mM Calcium chloride, 0.01 mM Iron (II) sulfate heptahydrate, 0.008 mM EDTA
Growth plate: 1524 A1

Specific Phenotypes

For 6 genes in this experiment

For nitrogen source L-Arginine in Caulobacter crescentus NA1000

For nitrogen source L-Arginine across organisms

SEED Subsystems

Subsystem	#Specific
Copper homeostasis	2
Cobalamin synthesis	1
Coenzyme B12 biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Porphyrin and chlorophyll metabolism
• Puromycin biosynthesis
• gamma-Hexachlorocyclohexane degradation
• Fluorobenzoate degradation
• Nucleotide sugars metabolism
• alpha-Linolenic acid metabolism
• 1,4-Dichlorobenzene degradation
• Trinitrotoluene degradation
• Riboflavin metabolism
• Carotenoid biosynthesis - General
• Alkaloid biosynthesis I
• Insect hormone biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
5-methoxy-6-methylbenzimidazolyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP	3	1	1
adeninyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP	3	1	1
5-hydroxybenzimidazolyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP	3	1	1
5-methoxybenzimidazolyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP	3	1	1
adenosylcobalamin biosynthesis from adenosylcobinamide-GDP I	3	1	1
5-methylbenzimidazolyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP	3	1	1
2-methyladeninyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP	3	1	1
benzimidazolyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP	3	1	1
4-chlorocatechol degradation	5	2	1
3-chlorocatechol degradation I (ortho)	5	1	1
3-chlorocatechol degradation II (ortho)	5	1	1
4,5-dichlorocatechol degradation	7	3	1
superpathway of adenosylcobalamin salvage from cobinamide I	8	4	1
3,5-dichlorocatechol degradation	8	3	1
3,4,6-trichlorocatechol degradation	9	4	1
superpathway of adenosylcobalamin salvage from cobinamide II	9	4	1
1,4-dichlorobenzene degradation	9	3	1
adenosylcobalamin biosynthesis II (aerobic)	33	15	1
adenosylcobalamin biosynthesis I (anaerobic)	36	14	1