Experiment set1IT051 for Caulobacter crescentus NA1000

D-Mannose carbon source

Group: carbon source
Media: M2_noCarbon + D-Mannose (20 mM)
Culturing: Caulo_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 5.3 generations
By: Jayashree on 22-Mar-17
Media components: 1.74 g/L Disodium phosphate, 1.06 g/L Potassium phosphate monobasic, 0.5 g/L Ammonium chloride, 0.5 mM Magnesium sulfate, 0.5 mM Calcium chloride, 0.01 mM Iron (II) sulfate heptahydrate, 0.008 mM EDTA
Growth plate: 1523 D1

Specific Phenotypes

For 2 genes in this experiment

For carbon source D-Mannose in Caulobacter crescentus NA1000

For carbon source D-Mannose across organisms

SEED Subsystems

Subsystem	#Specific
Biotin biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Fructose and mannose metabolism
• Starch and sucrose metabolism
• Nucleotide sugars metabolism
• Biotin metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
D-mannose degradation II	2	2	1
D-sorbitol degradation I	3	2	1
sucrose degradation I (sucrose phosphotransferase)	3	1	1
biotin biosynthesis from 8-amino-7-oxononanoate I	4	4	1
sucrose degradation IV (sucrose phosphorylase)	4	4	1
mannitol degradation II	4	3	1
biotin biosynthesis from 8-amino-7-oxononanoate II	4	3	1
sucrose degradation III (sucrose invertase)	4	3	1
sucrose degradation VII (sucrose 3-dehydrogenase)	4	2	1
CMP-2-keto-3-deoxy-D-glycero-D-galacto-nononate biosynthesis	4	1	1
sucrose degradation II (sucrose synthase)	5	4	1
1,5-anhydrofructose degradation	5	3	1
biotin biosynthesis from 8-amino-7-oxononanoate III	5	3	1
mannitol cycle	5	2	1
biotin biosynthesis II	6	4	1
biotin biosynthesis I	15	13	1
superpathway of CMP-sialic acids biosynthesis	15	3	1
heterolactic fermentation	18	12	1
superpathway of anaerobic sucrose degradation	19	14	1