Experiment set1IT048 for Caulobacter crescentus NA1000

N-Acetyl-D-Glucosamine carbon source

Group: carbon source
Media: M2_noCarbon + N-Acetyl-D-Glucosamine (20 mM)
Culturing: Caulo_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 5.8 generations
By: Jayashree on 22-Mar-17
Media components: 1.74 g/L Disodium phosphate, 1.06 g/L Potassium phosphate monobasic, 0.5 g/L Ammonium chloride, 0.5 mM Magnesium sulfate, 0.5 mM Calcium chloride, 0.01 mM Iron (II) sulfate heptahydrate, 0.008 mM EDTA
Growth plate: 1523 C4

Specific Phenotypes

For 7 genes in this experiment

For carbon source N-Acetyl-D-Glucosamine in Caulobacter crescentus NA1000

For carbon source N-Acetyl-D-Glucosamine across organisms

SEED Subsystems

Subsystem	#Specific
Chitin and N-acetylglucosamine utilization	3
Biotin biosynthesis	1
Glutathione: Non-redox reactions	1
Ketoisovalerate oxidoreductase	1
N-Acetyl-Galactosamine and Galactosamine Utilization	1
Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate	1
Ton and Tol transport systems	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycolysis / Gluconeogenesis
• Glutathione metabolism
• Other glycan degradation
• Nucleotide sugars metabolism
• Aminosugars metabolism
• Glycosaminoglycan degradation
• Glycosphingolipid biosynthesis - globo series
• Glycosphingolipid biosynthesis - ganglio series
• Pyruvate metabolism
• Propanoate metabolism
• Reductive carboxylate cycle (CO2 fixation)
• Biotin metabolism
• Metabolism of xenobiotics by cytochrome P450
• Drug metabolism - cytochrome P450

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
acetate conversion to acetyl-CoA	1	1	1
acetate and ATP formation from acetyl-CoA III	1	1	1
ethanol degradation II	3	3	1
ethanol degradation IV	3	3	1
ethanol degradation III	3	2	1
L-isoleucine biosynthesis V	3	2	1
chitin degradation II (Vibrio)	6	3	2
superpathway of acetate utilization and formation	3	1	1
biotin biosynthesis from 8-amino-7-oxononanoate I	4	4	1
chitin deacetylation	4	2	1
4-hydroxy-2-nonenal detoxification	4	1	1
pentachlorophenol degradation	10	3	2
2-methylcitrate cycle I	5	1	1
β-alanine biosynthesis II	6	4	1
L-isoleucine biosynthesis IV	6	4	1
superpathway of bitter acids biosynthesis	18	3	3
adlupulone and adhumulone biosynthesis	6	1	1
2-methylcitrate cycle II	6	1	1
colupulone and cohumulone biosynthesis	6	1	1
lupulone and humulone biosynthesis	6	1	1
chitin degradation III (Serratia)	7	3	1
glutathione-mediated detoxification I	8	3	1
reductive glycine pathway of autotrophic CO2 fixation	9	4	1
gliotoxin biosynthesis	9	2	1
glutathione-mediated detoxification II	9	1	1
cis-geranyl-CoA degradation	9	1	1
superpathway of coenzyme A biosynthesis II (plants)	10	8	1
peptidoglycan recycling II	10	2	1
indole glucosinolate activation (intact plant cell)	12	3	1
camalexin biosynthesis	12	2	1
peptidoglycan recycling I	14	8	1
biotin biosynthesis I	15	13	1