Experiment set1IT045 for Burkholderia phytofirmans PsJN

LB with Bacitracin 0.25 mg/ml

Group: stress
Media: LB + Bacitracin (0.25 mg/ml)
Culturing: BFirm_ML3, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
Growth: about 3.9 generations
By: Mark on 6/2/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: BFirm_SP4 C5

Specific Phenotypes

For 12 genes in this experiment

For stress Bacitracin in Burkholderia phytofirmans PsJN

For stress Bacitracin across organisms

SEED Subsystems

Subsystem	#Specific
Flagellum	2
Choline and Betaine Uptake and Betaine Biosynthesis	1
Isoprenoid Biosynthesis	1
Proteolysis in bacteria, ATP-dependent	1
Trehalose Biosynthesis	1
Ubiquinone Biosynthesis	1
YjeE	1
polyprenyl synthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycine, serine and threonine metabolism
• Starch and sucrose metabolism
• Nucleotide sugars metabolism
• Terpenoid biosynthesis
• Biosynthesis of terpenoids and steroids
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
trehalose biosynthesis I	2	2	1
glycine betaine biosynthesis III (plants)	3	3	1
CDP-4-dehydro-3,6-dideoxy-D-glucose biosynthesis	3	1	1
methylerythritol phosphate pathway II	9	8	2
methylerythritol phosphate pathway I	9	7	2
isoprene biosynthesis I	10	7	2
superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP)	12	10	2
taxadiene biosynthesis (engineered)	13	10	2
superpathway of CDP-glucose-derived O-antigen building blocks biosynthesis	8	1	1
superpathway of ergosterol biosynthesis II	26	11	2