Experiment set1IT043 for Caulobacter crescentus NA1000
m-Inositol carbon source
Group: carbon sourceMedia: M2_noCarbon + m-Inositol (20 mM)
Culturing: Caulo_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 4.4 generations
By: Jayashree on 22-Mar-17
Media components: 1.74 g/L Disodium phosphate, 1.06 g/L Potassium phosphate monobasic, 0.5 g/L Ammonium chloride, 0.5 mM Magnesium sulfate, 0.5 mM Calcium chloride, 0.01 mM Iron (II) sulfate heptahydrate, 0.008 mM EDTA
Growth plate: 1523 B3
Specific Phenotypes
For 5 genes in this experiment
For carbon source m-Inositol in Caulobacter crescentus NA1000
For carbon source m-Inositol across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Inositol catabolism | 2 |
| Biotin biosynthesis | 1 |
| D-ribose utilization | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Valine, leucine and isoleucine degradation
- Inositol phosphate metabolism
- Propanoate metabolism
- Biotin metabolism
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| β-alanine degradation II | 2 | 2 | 1 |
| β-alanine degradation I | 2 | 1 | 1 |
| biotin biosynthesis from 8-amino-7-oxononanoate I | 4 | 4 | 1 |
| biotin biosynthesis from 8-amino-7-oxononanoate II | 4 | 3 | 1 |
| biotin biosynthesis from 8-amino-7-oxononanoate III | 5 | 3 | 1 |
| propanoyl-CoA degradation II | 5 | 3 | 1 |
| biotin biosynthesis II | 6 | 4 | 1 |
| myo-inositol degradation I | 7 | 7 | 1 |
| 2,4-dinitrotoluene degradation | 7 | 1 | 1 |
| L-valine degradation I | 8 | 5 | 1 |
| myo-, chiro- and scyllo-inositol degradation | 10 | 7 | 1 |
| biotin biosynthesis I | 15 | 13 | 1 |