Experiment set1IT040 for Pseudomonas fluorescens FW300-N2E2

LB with Cisplatin 0.008 mg/ml

Group: stress
Media: LB + Cisplatin (0.008 mg/ml)
Culturing: pseudo6_N2E2_ML5, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Mark on 3/9/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 1181 E7,E8

Specific Phenotypes

For 18 genes in this experiment

For stress Cisplatin in Pseudomonas fluorescens FW300-N2E2

For stress Cisplatin across organisms

SEED Subsystems

Subsystem	#Specific
DNA-replication	3
DNA repair, UvrABC system	3
DNA Repair Base Excision	2
DNA repair, bacterial	2
DNA repair, bacterial DinG and relatives	2
DNA repair, bacterial RecFOR pathway	2
Methionine Salvage	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Methionine metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
5'-deoxyadenosine degradation I	3	1	1
5'-deoxyadenosine degradation II	4	1	1
S-methyl-5-thio-α-D-ribose 1-phosphate degradation II	5	2	1
S-methyl-5-thio-α-D-ribose 1-phosphate degradation III	5	2	1
S-methyl-5-thio-α-D-ribose 1-phosphate degradation I	7	2	1
L-methionine salvage cycle III	11	5	1
L-methionine salvage cycle II (plants)	11	3	1
L-methionine salvage cycle I (bacteria and plants)	12	4	1