Experiment set1IT039 for Pseudomonas fluorescens FW300-N2C3

LB with Cobalt chloride hexahydrate 0.08 mM

Group: stress
Media: LB + Cobalt chloride hexahydrate (0.08 mM)
Culturing: pseudo5_N2-C3_1_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Jayashree on 4/28/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 893 A7,A8

Specific Phenotypes

For 16 genes in this experiment

For stress Cobalt chloride hexahydrate in Pseudomonas fluorescens FW300-N2C3

For stress Cobalt chloride hexahydrate across organisms

SEED Subsystems

Subsystem	#Specific
Conserved gene cluster possibly involved in RNA metabolism	1
Copper homeostasis: copper tolerance	1
Cysteine Biosynthesis	1
Glycine and Serine Utilization	1
Methionine Biosynthesis	1
Periplasmic disulfide interchange	1
Phosphate metabolism	1
Pyruvate Alanine Serine Interconversions	1
Threonine anaerobic catabolism gene cluster	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Lipopolysaccharide biosynthesis
• Cysteine metabolism
• Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
• Sphingolipid metabolism
• Carotenoid biosynthesis - General
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Fructose and mannose metabolism
• Ubiquinone and menaquinone biosynthesis
• Puromycin biosynthesis
• Lysine biosynthesis
• Histidine metabolism
• Tyrosine metabolism
• Tryptophan metabolism
• Benzoxazinone biosynthesis
• Glutathione metabolism
• N-Glycan biosynthesis
• O-Glycan biosynthesis
• High-mannose type N-glycan biosynthesis
• O-Mannosyl glycan biosynthesis
• Nucleotide sugars metabolism
• Keratan sulfate biosynthesis
• Peptidoglycan biosynthesis
• Glycerolipid metabolism
• Glycosphingolipid biosynthesis - lacto and neolacto series
• Glycosphingolipid biosynthesis - globo series
• Glycosphingolipid biosynthesis - ganglio series
• Naphthalene and anthracene degradation
• 1,4-Dichlorobenzene degradation
• Nicotinate and nicotinamide metabolism
• Porphyrin and chlorophyll metabolism
• Zeatin biosynthesis
• Sulfur metabolism
• Caprolactam degradation
• Phenylpropanoid biosynthesis
• Flavone and flavonol biosynthesis
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Drug metabolism - other enzymes
• Biosynthesis of alkaloids derived from shikimate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
poly-β-1,6-N-acetyl-D-glucosamine biosynthesis	4	3	3
D-serine degradation	3	3	2
L-cysteine degradation II	3	3	2
L-serine degradation	3	3	2
L-tryptophan degradation II (via pyruvate)	3	2	2
L-cysteine biosynthesis I	2	2	1
felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis	5	2	2
L-methionine biosynthesis II	6	5	2
glycine betaine degradation III	7	7	2
glycine betaine degradation I	8	6	2
L-cysteine biosynthesis VII (from S-sulfo-L-cysteine)	4	3	1
L-mimosine degradation	8	4	2
glutathione-mediated detoxification I	8	3	2
N-3-oxalyl-L-2,3-diaminopropanoate biosynthesis	4	1	1
seleno-amino acid biosynthesis (plants)	5	3	1
D-cycloserine biosynthesis	6	1	1
L-cysteine biosynthesis VI (reverse transsulfuration)	7	3	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II	15	13	2
superpathway of sulfate assimilation and cysteine biosynthesis	9	9	1
purine nucleobases degradation II (anaerobic)	24	16	2
superpathway of seleno-compound metabolism	19	8	1