Experiment set1IT038 for Pseudomonas fluorescens FW300-N2E2

LB with Paraquat dichloride 0.03 mg/ml

Group: stress
Media: LB + Paraquat dichloride (0.03 mg/ml)
Culturing: pseudo6_N2E2_ML5, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Mark on 3/9/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 1181 D1,D2

Specific Phenotypes

For 3 genes in this experiment

For stress Paraquat dichloride in Pseudomonas fluorescens FW300-N2E2

For stress Paraquat dichloride across organisms

SEED Subsystems

Subsystem	#Specific
Lysine degradation	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glutamate metabolism
• Alanine and aspartate metabolism
• Lysine degradation
• beta-Alanine metabolism
• Propanoate metabolism
• Butanoate metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
4-aminobutanoate degradation III	2	2	1
4-aminobutanoate degradation II	2	2	1
4-aminobutanoate degradation I	2	1	1
superpathway of 4-aminobutanoate degradation	3	2	1
GABA shunt II	4	2	1
GABA shunt I	4	1	1
L-lysine degradation IV	5	5	1
L-lysine degradation X	6	5	1
L-lysine degradation III	6	2	1
L-lysine degradation I	7	4	1
4-aminobutanoate degradation V	7	2	1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation	11	9	1
superpathway of L-arginine and L-ornithine degradation	13	11	1
nicotine degradation I (pyridine pathway)	17	5	1
superpathway of L-lysine degradation	43	19	1