Experiment set1IT038 for Pseudomonas fluorescens FW300-N2C3

LB with Nickel (II) chloride hexahydrate 0.625 mM

Group: stress
Media: LB + Nickel (II) chloride hexahydrate (0.625 mM)
Culturing: pseudo5_N2-C3_1_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Jayashree on 4/28/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 893 A3,A4

Specific Phenotypes

For 14 genes in this experiment

For stress Nickel (II) chloride hexahydrate in Pseudomonas fluorescens FW300-N2C3

For stress Nickel (II) chloride hexahydrate across organisms

SEED Subsystems

Subsystem	#Specific
Alginate metabolism	1
Conserved gene cluster possibly involved in RNA metabolism	1
Copper homeostasis: copper tolerance	1
Cysteine Biosynthesis	1
CytR regulation	1
Mannose Metabolism	1
Methionine Biosynthesis	1
Phosphate metabolism	1
Quorum sensing regulation in Pseudomonas	1
cAMP signaling in bacteria	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Lipopolysaccharide biosynthesis
• Fructose and mannose metabolism
• Nucleotide sugars metabolism
• Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
• Sphingolipid metabolism
• Puromycin biosynthesis
• Cysteine metabolism
• Lysine biosynthesis
• Glutathione metabolism
• N-Glycan biosynthesis
• O-Glycan biosynthesis
• High-mannose type N-glycan biosynthesis
• O-Mannosyl glycan biosynthesis
• Keratan sulfate biosynthesis
• Peptidoglycan biosynthesis
• Glycerolipid metabolism
• Glycosphingolipid biosynthesis - lacto and neolacto series
• Glycosphingolipid biosynthesis - globo series
• Glycosphingolipid biosynthesis - ganglio series
• 1,4-Dichlorobenzene degradation
• Nicotinate and nicotinamide metabolism
• Carotenoid biosynthesis - General
• Zeatin biosynthesis
• Sulfur metabolism
• Caprolactam degradation
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Flavone and flavonol biosynthesis
• Drug metabolism - other enzymes

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
poly-β-1,6-N-acetyl-D-glucosamine biosynthesis	4	3	3
L-cysteine biosynthesis I	2	2	1
D-mannose degradation I	2	1	1
D-mannose degradation II	2	1	1
β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation	3	2	1
mannitol biosynthesis	3	1	1
GDP-mannose biosynthesis	4	4	1
L-cysteine biosynthesis VII (from S-sulfo-L-cysteine)	4	3	1
mannitol degradation II	4	2	1
N-3-oxalyl-L-2,3-diaminopropanoate biosynthesis	4	1	1
seleno-amino acid biosynthesis (plants)	5	3	1
1,5-anhydrofructose degradation	5	2	1
D-cycloserine biosynthesis	6	1	1
L-cysteine biosynthesis VI (reverse transsulfuration)	7	3	1
β-(1,4)-mannan degradation	7	2	1
superpathway of sulfate assimilation and cysteine biosynthesis	9	9	1
colanic acid building blocks biosynthesis	11	9	1
superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis	14	7	1
superpathway of seleno-compound metabolism	19	8	1