Experiment set1IT037 for Pedobacter sp. GW460-11-11-14-LB5

R2A with Cobalt chloride hexahydrate 0.08 mM

Group: stress
Media: R2A + Cobalt chloride hexahydrate (0.08 mM)
Culturing: Pedo557_ML3, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 4.6 generations
By: Adam on 17-May-17
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate
Growth plate: 1754 A5

Specific Phenotypes

For 11 genes in this experiment

For stress Cobalt chloride hexahydrate in Pedobacter sp. GW460-11-11-14-LB5

For stress Cobalt chloride hexahydrate across organisms

SEED Subsystems

Subsystem	#Specific
Cobalt-zinc-cadmium resistance	2
Copper homeostasis: copper tolerance	1
DNA repair, bacterial MutL-MutS system	1
Histidine Degradation	1
Phosphate metabolism	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Histidine metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-histidine degradation I	4	3	1
L-histidine degradation II	5	2	1
L-histidine degradation III	6	3	1
L-histidine degradation VI	8	6	1