Experiment set1IT036 for Rhodanobacter denitrificans FW104-10B01
R2A with Manganese (II) chloride tetrahydrate 2.5 mM
Group: stressMedia: R2A + Manganese (II) chloride tetrahydrate (2.5 mM)
Culturing: rhodanobacter_10B01_ML12, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Trenton on 1/24/21
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate
Specific Phenotypes
For 6 genes in this experiment
For stress Manganese (II) chloride tetrahydrate in Rhodanobacter denitrificans FW104-10B01
For stress Manganese (II) chloride tetrahydrate across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Glycine, serine and threonine metabolism
- Urea cycle and metabolism of amino groups
- Purine metabolism
- Arginine and proline metabolism
- Lipopolysaccharide biosynthesis
- One carbon pool by folate
- Folate biosynthesis
- Nitrogen metabolism
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| glycine cleavage | 3 | 3 | 2 |
| glycine biosynthesis II | 3 | 3 | 2 |
| glycine degradation | 3 | 3 | 1 |
| L-ornithine biosynthesis I | 5 | 5 | 1 |
| L-arginine biosynthesis III (via N-acetyl-L-citrulline) | 9 | 9 | 1 |
| folate transformations III (E. coli) | 9 | 9 | 1 |
| L-arginine biosynthesis I (via L-ornithine) | 9 | 8 | 1 |
| photorespiration III | 9 | 5 | 1 |
| reductive glycine pathway of autotrophic CO2 fixation | 9 | 5 | 1 |
| photorespiration I | 9 | 5 | 1 |
| photorespiration II | 10 | 5 | 1 |
| folate transformations II (plants) | 11 | 10 | 1 |
| folate transformations I | 13 | 8 | 1 |
| superpathway of arginine and polyamine biosynthesis | 17 | 12 | 1 |