Experiment set1IT036 for Pseudomonas fluorescens FW300-N2E2

Compare to:

LB with Cobalt chloride hexahydrate 0.16 mM

200 most detrimental genes:

  gene name fitness t score description  
Pf6N2E2_3192 +3.4 7.2 tRNA:Cm32/Um32 methyltransferase compare
Pf6N2E2_3191 +2.6 5.7 Serine acetyltransferase (EC 2.3.1.30) conserved
Pf6N2E2_2252 +2.5 7.8 NreA-like protein compare
Pf6N2E2_464 +1.9 7.4 Sensory histidine kinase QseC compare
Pf6N2E2_5420 +1.9 12.5 Cytosol aminopeptidase PepA (EC 3.4.11.1) compare
Pf6N2E2_4068 +1.9 16.5 Phosphate regulon metal ion transporter containing CBS domains compare
Pf6N2E2_5015 +1.6 9.6 Magnesium and cobalt efflux protein CorC compare
Pf6N2E2_3151 +1.6 8.6 Glutamate synthase [NADPH] small chain (EC 1.4.1.13) compare
Pf6N2E2_4803 +1.5 4.6 Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41) compare
Pf6N2E2_463 +1.5 10.7 Transcriptional regulatory protein RstA compare
Pf6N2E2_3942 +1.4 9.9 FIG001592: Phosphocarrier protein kinase/phosphorylase, nitrogen regulation associated compare
Pf6N2E2_2869 +1.3 2.2 Peptide deformylase (EC 3.5.1.88) compare
Pf6N2E2_3941 +1.2 3.7 Adenosine (5')-pentaphospho-(5'')-adenosine pyrophosphohydrolase (EC 3.6.1.-) compare
Pf6N2E2_2253 +1.1 1.5 hypothetical protein compare
Pf6N2E2_2384 +1.1 6.2 CmpX compare
Pf6N2E2_2254 +1.1 4.0 Cell filamentation protein fic compare
Pf6N2E2_4362 +1.1 4.2 DNA-directed RNA polymerase omega subunit (EC 2.7.7.6) compare
Pf6N2E2_2022 +1.1 6.3 BarA-associated response regulator UvrY (= GacA = SirA) compare
Pf6N2E2_4743 +1.1 2.1 hypothetical protein compare
Pf6N2E2_2465 +1.0 4.3 Cys regulon transcriptional activator CysB compare
Pf6N2E2_5178 +1.0 2.8 CDP-diacylglycerol--serine O-phosphatidyltransferase (EC 2.7.8.8) compare
Pf6N2E2_3152 +1.0 6.8 Magnesium and cobalt efflux protein CorC conserved
Pf6N2E2_5720 +1.0 8.0 BarA sensory histidine kinase (= VarS = GacS) compare
Pf6N2E2_4057 +1.0 1.6 lipoprotein, putative compare
Pf6N2E2_2580 +1.0 3.5 Flagellar biosynthesis protein FliP compare
Pf6N2E2_2675 +0.9 1.6 ATP-dependent RNA helicase SrmB compare
Pf6N2E2_4414 +0.9 3.0 FIG00953808: hypothetical protein compare
Pf6N2E2_5274 +0.9 6.1 FolM Alternative dihydrofolate reductase 1 conserved
Pf6N2E2_5030 +0.9 5.3 Transcriptional regulator, AsnC family compare
Pf6N2E2_987 +0.9 1.9 hypothetical protein compare
Pf6N2E2_533 +0.9 3.7 Na(+) H(+) antiporter subunit G compare
Pf6N2E2_5646 +0.9 2.3 PROBABLE MEMBRANE PROTEIN compare
Pf6N2E2_1596 +0.8 1.4 Periplasmic nitrate reductase component NapD compare
Pf6N2E2_405 +0.8 1.6 hypothetical protein compare
Pf6N2E2_2627 +0.8 4.0 Flagellar basal-body rod protein FlgG compare
Pf6N2E2_5695 +0.8 5.0 Flagellar basal-body rod protein FlgC compare
Pf6N2E2_1391 +0.8 2.9 DNA-binding response regulator, LuxR family compare
Pf6N2E2_1966 +0.8 2.5 SAM-dependent methyltransferases compare
Pf6N2E2_5694 +0.8 5.5 Flagellar basal-body rod protein FlgB compare
Pf6N2E2_1534 +0.8 2.0 Probable transmembrane protein compare
Pf6N2E2_5156 +0.8 2.0 Two-component sensor CbrB: intrcellular carbon:nitrogen balance compare
Pf6N2E2_3940 +0.8 6.1 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_988 +0.8 3.1 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27) compare
Pf6N2E2_2827 +0.8 1.7 hypothetical protein compare
Pf6N2E2_1210 +0.8 5.0 putative aminotransferase compare
Pf6N2E2_4148 +0.8 5.1 Glutamate-aspartate carrier protein compare
Pf6N2E2_3918 +0.7 4.0 D-cysteine desulfhydrase (EC 4.4.1.15) conserved
Pf6N2E2_2296 +0.7 1.6 FIG00954767: hypothetical protein compare
Pf6N2E2_4754 +0.7 6.5 Protein rarD compare
Pf6N2E2_2181 +0.7 2.1 Arginine N-succinyltransferase (EC 2.3.1.109) compare
Pf6N2E2_1089 +0.7 1.4 Delta 1-piperideine-2-carboxylate reductase (EC 1.5.1.21) / Delta 1-pyrroline-2-carboxylate reductase (EC 1.5.1.1) compare
Pf6N2E2_1506 +0.7 2.7 Putative alkanesulfonate metabolism utilization regulator compare
Pf6N2E2_2278 +0.7 6.2 Transcription-repair coupling factor compare
Pf6N2E2_5014 +0.7 1.5 Apolipoprotein N-acyltransferase (EC 2.3.1.-) / Copper homeostasis protein CutE compare
Pf6N2E2_1248 +0.7 2.2 MutT/nudix family protein compare
Pf6N2E2_2577 +0.7 5.1 Flagellar biosynthesis protein FlhB compare
Pf6N2E2_2597 +0.7 4.8 Flagellar regulatory protein FleQ compare
Pf6N2E2_4168 +0.7 4.9 Zinc ABC transporter, inner membrane permease protein ZnuB compare
Pf6N2E2_2591 +0.7 3.1 Flagellar assembly protein FliH compare
Pf6N2E2_5748 +0.7 3.1 Transcriptional activator protein LuxR compare
Pf6N2E2_923 +0.7 2.1 Haloacid dehalogenase, type II (EC 3.8.1.2) compare
Pf6N2E2_2471 +0.7 4.8 Glutamate synthase [NADPH] large chain (EC 1.4.1.13) compare
Pf6N2E2_5272 +0.7 3.2 Dihydroneopterin triphosphate epimerase compare
Pf6N2E2_2590 +0.7 4.5 Flagellum-specific ATP synthase FliI compare
Pf6N2E2_4943 +0.7 1.6 PspA/IM30 family protein compare
Pf6N2E2_3664 +0.7 4.2 Carbamoyltransferase in large core OS assembly cluster compare
Pf6N2E2_4918 +0.7 2.8 Ribonucleotide reductase transcriptional regulator NrdR compare
Pf6N2E2_3663 +0.7 3.8 Glycosyl transferase compare
Pf6N2E2_2594 +0.7 4.3 Flagellar hook-basal body complex protein FliE compare
Pf6N2E2_5340 +0.7 2.1 Stringent starvation protein A compare
Pf6N2E2_342 +0.7 1.5 DNA-binding response regulator compare
Pf6N2E2_3624 +0.6 4.3 Flagellar motor rotation protein MotB compare
Pf6N2E2_1676 +0.6 2.2 conserved protein associated with acetyl-CoA C-acyltransferase compare
Pf6N2E2_3014 +0.6 1.2 P2-like prophage tail protein X compare
Pf6N2E2_1974 +0.6 2.6 FIG141694: hypothetical protein in PFGI-1-like cluster compare
Pf6N2E2_5020 +0.6 3.1 FIG00955339: hypothetical protein compare
Pf6N2E2_2602 +0.6 4.2 Flagellin protein FlaA compare
Pf6N2E2_1340 +0.6 1.9 major pilin protein fimA compare
Pf6N2E2_2267 +0.6 2.0 Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-) compare
Pf6N2E2_2277 +0.6 1.7 NADPH-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.13) compare
Pf6N2E2_2152 +0.6 0.9 hypothetical protein compare
Pf6N2E2_1014 +0.6 1.1 Nitrate/nitrite transporter compare
Pf6N2E2_1499 +0.6 2.1 ABC transporter amino acid-binding protein compare
Pf6N2E2_1762 +0.6 2.2 putative lipase compare
Pf6N2E2_5705 +0.6 1.6 Sensor protein PhoQ (EC 2.7.13.3) compare
Pf6N2E2_2081 +0.6 1.3 putative exported protein compare
Pf6N2E2_3634 +0.6 1.1 Transporter compare
Pf6N2E2_2314 +0.6 2.0 VapB protein (antitoxin to VapC) compare
Pf6N2E2_1981 +0.6 3.2 FIG051360: Periplasmic protein TonB, links inner and outer membranes compare
Pf6N2E2_6047 +0.6 1.5 hypothetical protein compare
Pf6N2E2_2080 +0.6 1.2 hypothetical protein compare
Pf6N2E2_3499 +0.6 2.4 Permeases of the major facilitator superfamily compare
Pf6N2E2_2576 +0.6 4.7 Flagellar biosynthesis protein FlhA compare
Pf6N2E2_4343 +0.6 3.7 Phosphoprotein phosphatase PppA compare
Pf6N2E2_1463 +0.6 1.2 Isochorismate pyruvate-lyase (EC 4.-.-.-) compare
Pf6N2E2_304 +0.6 2.0 Anthranilate phosphoribosyltransferase like (EC 2.4.2.18) compare
Pf6N2E2_521 +0.6 1.5 hypothetical protein compare
Pf6N2E2_3665 +0.6 0.9 serine/threonine protein kinase compare
Pf6N2E2_2402 +0.6 1.7 hypothetical protein compare
Pf6N2E2_2592 +0.6 2.6 Flagellar motor switch protein FliG compare
Pf6N2E2_2623 +0.6 4.6 Flagellar hook-associated protein FlgK compare
Pf6N2E2_2585 +0.6 4.1 Flagellar hook-length control protein FliK compare
Pf6N2E2_3616 +0.6 4.0 DNA mismatch repair protein MutL compare
Pf6N2E2_5063 +0.6 4.0 tRNA (Uracil54-C5-)-methyltransferase (EC 2.1.1.35) conserved
Pf6N2E2_1834 +0.6 1.7 Enoyl-CoA hydratase (EC 4.2.1.17) compare
Pf6N2E2_2121 +0.6 2.2 hypothetical protein compare
Pf6N2E2_2795 +0.6 1.2 putative exported protein compare
Pf6N2E2_3980 +0.6 4.4 Type I secretion system, outer membrane component LapE compare
Pf6N2E2_1283 +0.6 1.5 Ferric siderophore transport system, periplasmic binding protein TonB compare
Pf6N2E2_3114 +0.6 3.9 Transcriptional regulator, TetR family compare
Pf6N2E2_5084 +0.6 4.1 Glutathione-regulated potassium-efflux system ATP-binding protein compare
Pf6N2E2_3144 +0.6 3.7 Thiol:disulfide interchange protein DsbC conserved
Pf6N2E2_5312 +0.5 1.7 FIG00964214: hypothetical protein compare
Pf6N2E2_4305 +0.5 0.7 FIG00959590: hypothetical protein compare
Pf6N2E2_4451 +0.5 2.7 FIG139438: lipoprotein B compare
Pf6N2E2_199 +0.5 1.7 hypothetical protein compare
Pf6N2E2_5794 +0.5 1.6 Ribosomal large subunit pseudouridine synthase A (EC 4.2.1.70) compare
Pf6N2E2_4630 +0.5 2.2 Ribosomal RNA small subunit methyltransferase E (EC 2.1.1.-) compare
Pf6N2E2_862 +0.5 2.4 Fumarylacetoacetate hydrolase family protein compare
Pf6N2E2_3625 +0.5 4.2 Flagellar motor rotation protein MotA compare
Pf6N2E2_4153 +0.5 1.0 Sensory box histidine kinase compare
Pf6N2E2_2017 +0.5 2.0 DNA-binding protein compare
Pf6N2E2_1896 +0.5 1.0 hypothetical protein compare
Pf6N2E2_865 +0.5 1.4 Fumarylacetoacetate hydrolase family protein compare
Pf6N2E2_1435 +0.5 2.1 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases; sulfonate monooxygenase compare
Pf6N2E2_70 +0.5 1.7 tRNA pseudouridine synthase A (EC 4.2.1.70) compare
Pf6N2E2_3885 +0.5 1.6 3'(2'),5'-bisphosphate nucleotidase (EC 3.1.3.7) compare
Pf6N2E2_4467 +0.5 1.0 hypothetical protein compare
Pf6N2E2_1136 +0.5 1.7 Transcriptional regulator, TetR family compare
Pf6N2E2_6077 +0.5 3.6 Cysteine desulfurase (EC 2.8.1.7) compare
Pf6N2E2_2110 +0.5 2.2 LysR family transcriptional regulator near succinyl-CoA:3-ketoacid-coenzyme A transferase compare
Pf6N2E2_1987 +0.5 1.5 hypothetical protein compare
Pf6N2E2_1567 +0.5 2.1 FIG00964846: hypothetical protein compare
Pf6N2E2_2583 +0.5 2.6 Flagellar motor switch protein FliM compare
Pf6N2E2_1162 +0.5 1.5 Transmembrane amino acid efflux protein compare
Pf6N2E2_2593 +0.5 2.8 Flagellar M-ring protein FliF compare
Pf6N2E2_2563 +0.5 4.1 FIG00953562: hypothetical protein compare
Pf6N2E2_2872 +0.5 3.8 Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) compare
Pf6N2E2_2067 +0.5 4.4 cis/trans isomerase conserved
Pf6N2E2_3285 +0.5 2.7 Rod shape-determining protein MreD compare
Pf6N2E2_3226 +0.5 1.6 Predicted D-glucarate or D-galactorate regulator, GntR family compare
Pf6N2E2_4990 +0.5 1.9 Uncharacterized protein, similar to the N-terminal domain of Lon protease compare
Pf6N2E2_2628 +0.5 2.0 Flagellar basal-body rod protein FlgF compare
Pf6N2E2_2579 +0.5 3.3 Flagellar biosynthesis protein FliQ compare
Pf6N2E2_1975 +0.5 1.8 Integrase regulator R compare
Pf6N2E2_4204 +0.5 0.9 rRNA small subunit 7-methylguanosine (m7G) methyltransferase GidB compare
Pf6N2E2_4010 +0.5 3.6 Fatty acid desaturase (EC 1.14.19.1); Delta-9 fatty acid desaturase (EC 1.14.19.1) compare
Pf6N2E2_1545 +0.5 2.2 N-carbamoylputrescine amidase (EC 3.5.1.53) compare
Pf6N2E2_2313 +0.5 2.1 Cytochrome c oxidase subunit CcoP (EC 1.9.3.1) compare
Pf6N2E2_2552 +0.5 3.4 Cytochrome c heme lyase subunit CcmH compare
Pf6N2E2_2213 +0.5 2.5 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf6N2E2_4659 +0.5 2.4 Lipoprotein, putative compare
Pf6N2E2_3204 +0.5 1.9 Rho-specific inhibitor of transcription termination (YaeO) compare
Pf6N2E2_1091 +0.5 1.6 Flavodoxin reductases (ferredoxin-NADPH reductases) family 1; Vanillate O-demethylase oxidoreductase (EC 1.14.13.-) compare
Pf6N2E2_2788 +0.5 1.6 Transcriptional regulator, AsnC family compare
Pf6N2E2_2578 +0.5 2.0 Flagellar biosynthesis protein FliR compare
Pf6N2E2_2622 +0.5 3.7 Flagellar hook-associated protein FlgL compare
Pf6N2E2_959 +0.5 1.7 putative membrane protein compare
Pf6N2E2_2519 +0.5 0.8 Biosynthetic Aromatic amino acid aminotransferase beta (EC 2.6.1.57) compare
Pf6N2E2_919 +0.5 0.8 FIG00954373: hypothetical protein compare
Pf6N2E2_449 +0.5 1.8 Lactoylglutathione lyase (EC 4.4.1.5) compare
Pf6N2E2_822 +0.5 2.6 TPR domain protein compare
Pf6N2E2_5697 +0.5 4.1 Flagellar hook protein FlgE compare
Pf6N2E2_5132 +0.5 3.4 NADH dehydrogenase (EC 1.6.99.3) conserved
Pf6N2E2_2734 +0.5 2.0 Transcriptional regulator, LysR family compare
Pf6N2E2_2352 +0.5 1.3 Type III secretion bridge between inner and outermembrane lipoprotein (YscJ,HrcJ,EscJ, PscJ) compare
Pf6N2E2_4331 +0.5 1.4 hypothetical protein compare
Pf6N2E2_291 +0.5 2.4 ATP-dependent Clp protease adaptor protein ClpS compare
Pf6N2E2_4028 +0.5 3.5 Biofilm PGA synthesis auxiliary protein PgaD conserved
Pf6N2E2_3875 +0.5 1.2 FIG00953324: hypothetical protein compare
Pf6N2E2_4534 +0.5 1.7 hypothetical protein compare
Pf6N2E2_1824 +0.5 1.4 hypothetical protein compare
Pf6N2E2_3475 +0.5 0.7 hypothetical protein compare
Pf6N2E2_3981 +0.5 4.1 T1SS secreted agglutinin RTX compare
Pf6N2E2_6035 +0.5 1.5 alpha/beta hydrolase fold compare
Pf6N2E2_1479 +0.5 2.5 Subtilisin-like serine proteases compare
Pf6N2E2_4263 +0.5 1.8 Putative metal chaperone, involved in Zn homeostasis, GTPase of COG0523 family compare
Pf6N2E2_3386 +0.4 2.0 hypothetical protein compare
Pf6N2E2_479 +0.4 1.8 Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase (EC 2.3.1.168) (from data) compare
Pf6N2E2_629 +0.4 1.9 2-ketogluconate kinase (EC 2.7.1.13) compare
Pf6N2E2_2323 +0.4 1.4 Fumarate and nitrate reduction regulatory protein compare
Pf6N2E2_6087 +0.4 1.5 Short-chain dehydrogenase/reductase SDR compare
Pf6N2E2_2175 +0.4 1.5 Transcriptional regulator, RpiR family compare
Pf6N2E2_3978 +0.4 3.7 Type I secretion system, membrane fusion protein LapC compare
Pf6N2E2_1480 +0.4 1.8 SAM-dependent methyltransferases compare
Pf6N2E2_6075 +0.4 1.8 Chromosome segregation ATPases compare
Pf6N2E2_2499 +0.4 1.1 Competence protein ComEA helix-hairpin-helix region precursor compare
Pf6N2E2_1968 +0.4 1.1 Cobalt-zinc-cadmium resistance protein compare
Pf6N2E2_2600 +0.4 3.6 Flagellar hook-associated protein FliD compare
Pf6N2E2_2089 +0.4 1.7 Transcriptional regulator, AraC family compare
Pf6N2E2_144 +0.4 3.8 Uncharacterized protein YtfM precursor compare
Pf6N2E2_2279 +0.4 1.5 FIG00954981: hypothetical protein compare
Pf6N2E2_3224 +0.4 2.0 Lactoylglutathione lyase and related lyases compare
Pf6N2E2_2472 +0.4 1.3 hypothetical protein compare
Pf6N2E2_945 +0.4 1.3 Transcriptional regulator, TetR family compare
Pf6N2E2_367 +0.4 1.6 Acetylornithine deacetylase (EC 3.5.1.16) compare
Pf6N2E2_3778 +0.4 1.7 FIG00954300: hypothetical protein compare
Pf6N2E2_5196 +0.4 3.1 Transcriptional regulator, Cro/CI family compare
Pf6N2E2_2791 +0.4 1.5 FIG00954883: hypothetical protein compare
Pf6N2E2_350 +0.4 1.3 Gfa-like protein compare


Specific Phenotypes

For 2 genes in this experiment

For stress Cobalt chloride hexahydrate in Pseudomonas fluorescens FW300-N2E2

For stress Cobalt chloride hexahydrate across organisms